| 陈 露 1, 谷少华 1, 王雄雅 1, 李显春 1, 2*.烟粉虱抗、感杀虫剂品系内生菌群落的差异分析[J].植物保护,2018,44(1):59-66. |
| 烟粉虱抗、感杀虫剂品系内生菌群落的差异分析 |
| Comparative analysis of bacterial community between insecticide- resistant and susceptible strains of Bemisia tabaci |
| 投稿时间：2017-04-16 修订日期：2017-04-24 |
| DOI：10.16688/j.zwbh.2017141 |
| 中文关键词: 烟粉虱 生物型 抗药性 16S rRNA测序 内共生菌 |
| 英文关键词:Bemisia tabaci biotype insecticide resistance 16S rRNA amplicon sequencing endosymbiont |
| 基金项目:北京市科技新星项目 (Z1511000003150118); 北京市青年拔尖个人项目 (2015000021223ZK29); 国家自然科学基金 (31772164, 31401737) |
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| 中文摘要: |
| 本研究对B型和Q型烟粉虱的吡虫啉或吡丙醚的抗、感品系进行16S rRNA细菌多样性测序分析, 以明确内共生菌的种类和数量与烟粉虱生物型和抗性的关系。结果表明Candidatus Nitrososphaera、Bacillus、Sphingobium 只在Q型烟粉虱中存在, 而Candidatus Hamiltonella、Lactobacillus、Helicobacter 等只在B型烟粉虱中存在, 说明这些内共生菌可能与烟粉虱B、Q两种生物型的分化有关; 内共生菌 Rickettsia、 Candidatus Portiera、 Candidatus Hamiltonella、S24-7、Prevotella 等在烟粉虱的新烟碱类杀虫剂吡虫啉抗性品系中明显多于敏感品系, 其可能与烟粉虱对吡虫啉的抗性有关; 在对保幼激素类似物杀虫剂吡丙醚的抗性品系中, Candidatus Hamiltonella -OTU7、 Candidatus Portiera -OTU5、 Candidatus Portiera -OTU190、 Rickettsia -OTU4、 Rickettsia -OTU2的数量明显多于敏感品系, 说明这些内共生菌可能与烟粉虱对该类药物的抗性有关。 |
| 英文摘要: |
| To study the relationships between biotypes and resistance of Bemisia tabaci and its endosymbionts, we compared the microbiota among different strains of B and Q biotypes that are susceptible or resistant to neonicotinoids orpypriproxyfen by using 16S rRNA amplicon sequencing technology. Candidatus Nitrososphaera, Bacillus and Sphingobsium were found in Q biotype strain only, whereas Candidatus Hamiltonella, Lactobacillus and Helicobacter were detected only in the B biotype strains. This suggested that these endosymbionts might be related to the differentiation of B/Q biotypes. Rickettsia, Candidatus Portiera, Candidatus Hamiltonella, S24-7 and Prevotella were significantly enriched in the imidacloprid resistant strains than in the susceptible strain, suggesting that these endosymbionts might contribute to B.tabaci ’s resistance to imidacloprid.Furthermore, the pyriproxyfen-resistant strain carried significantly more Candidatus Hamiltonella- OTU7, Candidatus Portiera- OTU5, Candidatus Portiera- OTU 190, Rickettsia- OTU 4 and Rickettsia- OTU 2 than the susceptible strain, implicating that these endosymbionts might be involved in B.tabaci ’s resistance to pyriproxyfen. Taken together, the data obtained support the involvements of endosymbionts in B/Q differentiation and their resistance to insecticides. |
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