| 申晓娜 1, 2, 吕志创 2, 李有志 1, 刘万学 2, 万方浩 2.温度胁迫下烟粉虱MED隐种与亚洲Ⅱ3隐种三个 抗寒基因表达模式的分析[J].植物保护,2018,44(1):52-58. |
| 温度胁迫下烟粉虱MED隐种与亚洲Ⅱ3隐种三个 抗寒基因表达模式的分析 |
| Comparative analysis of the expression patterns of three cold-resistant gene in MED and Asia Ⅱ3 cryptic species of Bemisia tabaci under temperature stress |
| 投稿时间：2017-05-23 修订日期：2017-06-29 |
| DOI：10.16688/j.zwbh.2017189 |
| 中文关键词: 烟粉虱 温度胁迫 抗寒基因 qRT-PCR 定量表达 |
| 英文关键词:Bemisia tabaci temperature stress cold-resistance gene qRT-PCR quantitative expression |
| 基金项目:国家自然科学基金 (31672088); 国家重点研发计划 (2016YFC1200603); 中以合作项目 (2015dfg32300); 国家公益性行业（农业）科研专项 (201303019) |
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| 中文摘要: |
| 烟粉虱 Bemisia tabaci 具有强的温度耐受性, 但烟粉虱入侵种MED隐种与本地种亚洲Ⅱ3隐种在我国的地理分布上存在巨大的差异。因此本研究以烟粉虱MED隐种和亚洲Ⅱ3隐种为研究对象, 采用qRT-PCR技术分别检测两种烟粉虱3个抗寒基因 (erythrocyte binding protein, trehalase, dopadecarboxylase) 在5个梯度温度胁迫下mRNA水平上的相对表达量, 分析其在高低温胁迫过程中表达量的变化, 探索抗寒基因在烟粉虱MED隐种成功入侵并广泛分布于各气候区域中所起的作用。研究结果表明这两种烟粉虱隐种体内, 3个抗寒基因均显著受低温诱导。但相对于AsiaⅡ3隐种, 低温胁迫下烟粉虱MED隐种体内的3种抗寒基因能更快速地做出响应, 且基因上调或下调的量也相对较多。 |
| 英文摘要: |
| Bemisia tabaci has strong temperature tolerance (heat resistance and cold resistance) . However, there were significant differences in the distribution of invasive species (MED cryptic species) and native species (Asia Ⅱ 3 cryptic species) in China. Therefore, in this study, we used qRT-PCR to detect the mRNA relative expression level of 3 important cold-resistant genes encoding the erythrocyte binding protein, trehalase and dopadecarboxylase in the two B. tabaci cryptic species under the stress of five gradient temperatures. The purpose of the study is to explore the roles of cold-resistant genes in the successful invasion of B. tabaci MED cryptic species and widespread distribution in different climatic regions by analyzing the change of the relative expression level. The results of qPCR test showed that three cold-resistant genes were significantly induced by low temperature in the two B. tabaci cryptic species. Compared with the Asia Ⅱ 3 cryptic species, B.tabaci MED could make adjustments to adapt to the low-temperature environment more quickly, and the adjustment level was also relatively higher. Therefore, we speculate that it may be one of the important mechanisms that makes MED cryptic species invade into a wide range of cold regions and adapt quickly to survive. |
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