| 黄大野1, 贲海燕2, 曹春霞1, 王开梅1, 饶犇1, 刘晓艳1*, 万中义1*, 杨自文1.死亡谷芽胞杆菌NBIF-001防治番茄棒孢叶斑病研究[J].植物保护,2017,43(6):192-195. |
| 死亡谷芽胞杆菌NBIF-001防治番茄棒孢叶斑病研究 |
| Control effect of Bacillus vallismortis NBIF-001 on Corynespora leaf spot of tomato |
| 投稿时间：2017-02-07 修订日期：2017-05-24 |
| DOI： |
| 中文关键词: 死亡谷芽胞杆菌 棒孢叶斑病菌 生防效果 |
| 英文关键词:Bacillus vallismortis Corynespora leaf spot biological control effect |
| 基金项目:湖北省自然科学基金重点项目(2016ABA103); 国家重点研发计划(SQ2017ZY060083); 湖北省农业科技创新中心创新团队项目(2016-620-000-001-038) |
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| 中文摘要: |
| 为明确死亡谷芽胞杆菌NBIF-001菌株对棒孢叶斑病的防治效果,采用平板对峙结合显微观察确定其对多主棒孢的抑菌活性,并采用活体盆栽试验研究该菌株对番茄棒孢叶斑病防治效果。结果表明,在平板对峙条件下NBIF-001对多主棒孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形; 盆栽试验结果表明 NBIF-001对棒孢叶斑病的防治效果达到71.46%。NBIF-001和50%啶酰菌胺WG联用防治番茄棒孢叶斑病的效果明显好于单独施用50%啶酰菌胺WG，具有较明显的增效作用,化学杀菌剂50%啶酰菌胺WG对NBIF-001菌株生长无明显影响,两者具有较好的相容性。 |
| 英文摘要: |
| In order to evaluate control effect of Bacillus vallismortis NBIF-001 on Corynespora leaf spot, the inhibition activity was tested by dual culture method and microscope observation, and the control efficacy was investigated by pot experiment. The results showed that the strain NBIF-001 had high inhibition activity in dual culture, hypha deformed and cytoplasmic vacuoles enlarged. Pot experiment demonstrated that NBIF-001 exhibited a better control effect on Corynespora leaf spot of tomato with the efficacy of 71.46%. NBIF001 combined with boscalid 50% WG had obvious synergic effect on the disease control. Bocalid showed no significant effect on the growth of NBIF-001, thus they both had the good compatibility. |
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