| 窦志敏1, 潘玲玲1, 邓权清1, 王凯莉1, 陈培寿1, 沈万宽1,2*.广东果蔗宿根矮化病菌检测[J].植物保护,2017,43(6):163-167. |
| 广东果蔗宿根矮化病菌检测 |
| Detection of Leifsonia xyli subsp. xyli, causal bacterium of sugarcane ratoon stunting disease, from fruit cane planting areas in Guangdong |
| 投稿时间：2017-02-23 修订日期：2017-04-19 |
| DOI： |
| 中文关键词: 果蔗 宿根矮化病 常规PCR 巢式PCR |
| 英文关键词:fruit cane ratoon stunting disease conventional PCR nested PCR |
| 基金项目:广州市科技计划项目(201604020087); 华南农业大学人才引进启动项目(13009) |
| 作者 | 单位 | | 窦志敏1, 潘玲玲1, 邓权清1, 王凯莉1, 陈培寿1, 沈万宽1,2* | 华南农业大学农学院, 农业部华南地区作物栽培科学观察实验站, 广州510642
2. 华南农业大学农学院, 广东省微生物信号与病害防控重点实验室, 广州510642 |
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| 中文摘要: |
| 为探明广东果蔗宿根矮化病发生情况,为果蔗健康种苗生产及推广应用提供科学依据,本研究采用常规PCR与巢式PCR技术分别对广东韶关和广州南沙果蔗产区的主栽品种‘黑皮果蔗’(‘Badila’)及华南农业大学甘蔗育种基地新引进的果蔗品种‘内江蜜蔗’、‘甜城21号’、‘甜城22号’和‘甜城99号’等进行宿根矮化病菌的检测。结果表明,巢式PCR检测的阳性检出率达88.6%,明显高于常规PCR检测(40.4%); 广东韶关果蔗产区‘黑皮果蔗’宿根矮化病阳性率为86.8%; 广州南沙果蔗产区‘黑皮果蔗’宿根矮化病阳性率为92.7%; 华南农业大学甘蔗育种基地新引进的果蔗品种除‘甜城22号’外,其他3个品种‘内江蜜蔗’、‘甜城21号’和‘甜城99号’均感染宿根矮化病菌。甘蔗宿根矮化病已在广东主要果蔗产区普遍发生,健康种苗研究与应用十分必要。 |
| 英文摘要: |
| This study aimed to understand the occurrence situation of ratoon stunting disease (RSD) in Guangdong fruit cane planting areas and provide scientific basis for the production and application of healthy seedlings. Conventional PCR and nested PCR were used to detect Leifsonia xyli subsp. xyli, the causal bacterium of ratoon stunting disease, from fruit cane cultivars, including ‘Badila’, ‘Neijiang mizhe’, ‘Tiancheng No.21’, ‘Tiancheng No.22’ and ‘Tiancheng No.99’. Samples of ‘Badila’ used as the main fruit cane cultivar were collected from Shaoguan City and Nansha District of Guangdong Province. Samples of introduced fruit cane cultivars ‘Neijiang mizhe’, ‘Tiancheng No.21’, ‘Tiancheng No.22’ and ‘Tiancheng No.99’ were collected from the sugarcane breeding base of South China Agricultural University (SCAU). The results showed that the positive detection rate was 88.6% by nested PCR, significantly higher than that by conventional PCR, which was 40.4%. The RSD positive rate of ‘Badila’ was 86.8% in Shaoguan and 92.7% in Nansha of Guangzhou, respectively. The introduced fruit cane cultivars from the sugarcane breeding base of SCAU were all infected by RSD pathogen except the ‘Tiancheng No.22’. The sugarcane ratoon stunting disease has occurred widely in the major fruit cane planting areas of Guangdong. It is necessary to carry out the research and application of healthy fruit cane seedlings. |
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