| 饶敏1, 桂家祥1, 卢占军2, 肖青青3, 张岑1.基于近红外技术柑橘黄龙病田间快速检测方法研究[J].植物保护,2017,43(6):135-138. |
| 基于近红外技术柑橘黄龙病田间快速检测方法研究 |
| Fast determination of Huanglongbing disease in the field by near infrared spectroscopy |
| 投稿时间：2017-02-23 修订日期：2017-05-23 |
| DOI： |
| 中文关键词: 近红外光谱技术 柑橘黄龙病 田间快速检测 PLS-LDA |
| 英文关键词:near infrared spectroscopy citrus Huanglongbing disease rapid detection in the field PLS-LDA |
| 基金项目:江西省科技计划项目(20152ACF60003, 20151BBG70067, 20141BBF60053); 江西省科研院所基础设施项目(20151BBA13045); 国家自然科学基金(31560602) |
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| 中文摘要: |
| 柑橘黄龙病在感病植株和健康植株之间传播速度快，因此快速检测柑橘黄龙病对其防治十分关键。本文研究了近红外技术快速检测柑橘黄龙病的方法。采用PLS-LDA建立的模型对未参与建模的样品进行了检测，结果表明，该模型检测的准确率与普通PCR检测的结果符合率达到100%，假阳性率小于1%。该技术具有检测周期短、无污染等优点，可用于田间黄龙病的快速检测。 |
| 英文摘要: |
| It is very important to develop rapid detection method for Huanglongbing (HLB) disease, because of the rapid spread of the disease. In this paper, the near infrared technique was applied for rapid detection of HLB. The model of PLS-LDA was used to detect the samples which did not participate in the modeling. The accurate rate of this technique was 100% same with that of PCR, and the false positive rate was less than 1%. Field detection showed that the near infrared technique for HLB rapid detection is feasible and practical, and can be used in the field detection of HLB disease, with the advantages of short detection cycle, no pollution, etc. |
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