| 刘荣荣, 王 暄, 李红梅*, 王 珍, 刘海璐.河南省小麦主产区菲利普孢囊线虫与禾谷孢囊线虫发生情况调查[J].植物保护,2017,43(5):157-163. |
| 河南省小麦主产区菲利普孢囊线虫与禾谷孢囊线虫发生情况调查 |
| Survey of the occurrence of Heterodera filipjevi and H.avenae on wheat in main growing area in Henan Province |
| 投稿时间：2016-12-10 修订日期：2017-03-29 |
| DOI： |
| 中文关键词: 菲利普孢囊线虫 禾谷孢囊线虫 复合侵染 群体密度 双重PCR检测 |
| 英文关键词:Heterodera filipjevi H.avenae co-infection population density duplex-PCR detection |
| 基金项目:公益性行业（农业）科研专项（201503114）；国家自然科学基金（31471751） |
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| 中文摘要: |
| 为了解河南省小麦主产区菲利普孢囊线虫与禾谷孢囊线虫的发生情况,作者分别于2014年和2015年5月通过随机采样调查方法,从商丘、漯河、许昌、新乡、鹤壁、安阳、濮阳、焦作等八地区19个县市采集到61份小麦孢囊线虫(CCN)的根围土壤样品,分析了样品的孢囊密度,发现采自商丘、新乡、鹤壁、安阳和濮阳地区的样品孢囊密度普遍较高,CCN发生比较严重; 利用双重PCR技术对样品的CCN种类进行了快速检测鉴定,检测出禾谷孢囊线虫单一侵染的样品有33份; 菲利普孢囊线虫单一侵染的样品有9份,与禾谷孢囊线虫复合侵染的样品有19份。菲利普孢囊线虫主要采自商丘、漯河、许昌、新乡、焦作等五个地区,其中商丘地区发生比较普遍。该研究可为河南省小麦的品种布局和孢囊线虫的综合防治提供指导。 |
| 英文摘要: |
| In order to clear the occurrence of Heterodera filipjevi and H.avenae on wheat in main growing area of Henan Province, China, we carried out the field survey by random sampling during May of year 2014 and 2015. Total 61 samples of cereal cyst nematodes (CCN) with root and soil were collected from 19 counties of 8 districts, including Shangqiu, Luohe, Xuchang, Xinxiang, Hebi, Anyang, Puyang and Jiaozuo, and the cyst densities of samples were analyzed. The results showed that the cyst densities of samples collected from district Shangqiu, Xinxiang, Hebi, Anyang and Puyang were higher and the CCN occurrence was severer than those from other three districts. The CCN species of samples were rapidly identified using the duplex-PCR technique. The detection analysis revealed that 33 samples were collected from wheat field solely infected with H.avenae, 9 samples solely infected with H.filipjevi, and 19 samples co-infected with H.filipjevi and H.avenae. H.filipjevi was mainly collected from Shangqiu, Luohe, Xuchang, Xinxiang and Jiaozuo districts and it was the most common CCN species in Shangqiu district. The study provided the useful information for distribution of wheat varieties cultivation and integrated control of CCN in Henan Province. |
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