| 赵兴华1, 于沛侠2, 陈丽君2, 王德富2, 牛颜冰2*.侵染芹菜的甜椒内源RNA病毒鉴定及序列分析[J].植物保护,2017,43(5):143-146. |
| 侵染芹菜的甜椒内源RNA病毒鉴定及序列分析 |
| Detection and sequence analysis of Bell pepper alphaendornavirus infecting Apium graveolens |
| 投稿时间：2016-10-25 修订日期：2017-01-10 |
| DOI： |
| 中文关键词: 芹菜 病原鉴定 甜椒内源RNA病毒 序列分析 |
| 英文关键词:Apium graveolens pathogen identification Bell pepper alphaendornavirus sequence analysis |
| 基金项目:公益性行业（农业）科研专项(201303028); 国家自然科学基金(31540050) |
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| 中文摘要: |
| 本试验利用双链RNA(double-stranded RNA, dsRNA)提取技术和非序列依赖PCR扩增(sequence-independent amplification，SIA)方法对表现皱缩症状的芹菜进行分子鉴定，发现芹菜被甜椒内源RNA病毒Bell pepper alphaendornavirus (BPEV)所侵染。为进一步明确山西芹菜BPEV分离物(BPEV-QC，GenBank登录号为KY659226)的分类地位，根据SIA测序结果设计BPEV特异性引物进行RT-PCR检测，并进行序列相似性比较和系统进化分析。序列同源性分析表明:BPEV-QC与BPEV分离物(IS、Maor、Kyosuzu、BPEV、lj、BPEV-YW)的同源性为80.0%～97.8%，与其他内源RNA病毒科分离物的同源性仅为30.6%～37.6%，表明:BPEV-QC与BPEV分离物(IS、Maor、Kyosuzu、BPEV、lj)形成一个独立分支，亲缘关系最近。 |
| 英文摘要: |
| Viral pathogen that caused Apium graveolens wrinkled disease, was identified by double-stranded RNA (dsRNA) extraction and sequence-independent amplification (SIA). The results of SIA and sequencing showed that A.graveolens was infected by Bell pepper alphaendornavirus （BPEV）. To further characterize the BPEV strain from A.graveolens (BPEV-QC), specific primer pairs were designed for RT-PCR according to the results of SIA.Sequence alignments showed that BPEV-QC shared high homology (80.0%-97.8%) with BPEV isolates (IS, Maor, Kyosuzu, BPEV, lj, BPEV-YW), but shared low homology (30.6%-37.6%) with other Endornaviridae isolates, Phylogenetic analysis based on nucleotide sequences of BPEV-QC and other Endornaviridae isolates showed that BPEV-QC was most closely related to Endornaviridae isolates (IS, Maor, Kyosuzu, BPEV, lj), and these isolates formed an independent branch. |
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