| 刘天波1,4, 周志成1, 彭曙光2, 曾维爱3, 唐前君4*.基于全基因组编码区序列的烟草花叶病毒分子进化分析[J].植物保护,2017,43(5):87-92. |
| 基于全基因组编码区序列的烟草花叶病毒分子进化分析 |
| Complete genomic coding sequence-based molecular evolutionary analysis of Tobacco mosaic virus |
| 投稿时间：2016-12-19 修订日期：2017-01-23 |
| DOI： |
| 中文关键词: 烟草花叶病毒 全基因组编码序列 系统发育 分子进化 |
| 英文关键词:Tobacco mosaic virus complete genomic coding sequence phylogenetics molecular evolution |
| 基金项目:中国烟草总公司湖南省公司科技项目专项(14-16ZDAa02); 中国烟草总公司科技重点项目(110201502016) |
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| 中文摘要: |
| 为揭示烟草花叶病毒分子进化特征，从GenBank中下载已报道的烟草花叶病毒全基因组编码区序列，进行重组、系统发育、遗传变异、种群结构和基因漂流等分析。结果表明:突变和负选择作用是驱动烟草花叶病毒进化的主要作用力，种群结构稳定，处于扩张趋势中，基因变异程度低，重组在烟草花叶病毒分子进化中作用不明显。烟草花叶病毒不同分离物形成3个有一定地理相关性的种群ChinaⅠ、China Ⅱ和Europe。欧洲分离物核苷酸序列多样性比中国的差异大，Europe和ChinaⅠ种群可能受到遗传漂变影响，Europe与China Ⅱ、ChinaⅠ与China Ⅱ之间存在发生基因交流的渠道。 |
| 英文摘要: |
| To reveal the molecular evolution of Tobacco mosaic virus (TMV), the complete genomic coding sequences of TMV from GenBank were subjected to molecular evolutionary analysis, including recombination, phylogeny, genetic variation, population structure, gene flow, etc. The results indicated that mutation and negative selection were the main driving forces for TMV evolution, rather than recombination. The TMV populations had stable structure, but were expanding, and the gene mutation was low. Three geographically correlative species of TMV named ChinaⅠ, China Ⅱand Europe were found. The Europe isolates exhibited greater nucleotide diversity than the China ones; Europe and ChinaⅠpopulations could be influenced by genetic drift, and there were gene exchange channels between Europe and China Ⅱ and between ChinaⅠand China Ⅱ. |
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