| 班一云, 丁 波*, 周雪平*.湖南省番茄和牵牛花上双生病毒的分子鉴定[J].植物保护,2017,43(4):134-138. |
| 湖南省番茄和牵牛花上双生病毒的分子鉴定 |
| Molecular identification of geminiviruses on tomato and morning glory in Hunan Province |
| 投稿时间：2017-03-25 修订日期：2017-04-27 |
| DOI： |
| 中文关键词: 番茄黄化曲叶病毒 甘薯卷叶病毒 DNA-A |
| 英文关键词:Tomato yellow leaf curl virus Sweet potato leaf curl virus DNA-A |
| 基金项目:国家自然科学基金重大项目(31390422) |
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| 中文摘要: |
| 双生病毒是一类在全世界范围内广泛发生的单链环状DNA病毒。本文对从湖南采集到的6例(洋姜、番茄、萝卜、赛葵、甘薯、牵牛花)疑似双生病毒侵染的植物叶片进行了分子鉴定。利用滚环扩增技术(RCA)对样品DNA进行扩增, 分别对其RCA产物进行酶切, 并将酶切得到的片段测序后进行BLAST比对, 结果显示番茄样品中的病毒分离物与番茄黄化曲叶病毒相似性最高(99％), 牵牛花样品中的病毒分离物与甘薯卷叶病毒相似性最高(99％), 证明这两个分离物是单组分DNA A双生病毒。这是在湖南省首次发现并报道双生病毒的全核酸序列。 |
| 英文摘要: |
| Geminiviruses including a group of single stranded circular DNA viruses occurred worldwide. Six plant samples collected from Hunan Province with symptoms of suspicious geminivirus infection were examined in this study. After conducting rolling circle amplification (RCA), the RCA products were digested by restriction endonuclease. Sequencing analysis revealed that virus isolates from tomato and morning glory shared 99% of homology with Tomato yellow leaf curl virus (TYLCV) and Sweet potato leaf curl virus (SPLCV), respectively. These results indicated that tomato and morning glory plants were infected by monopartite geminivirus, and this is the first report of geminivirus infection in Hunan Province |
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