| 尤佳琪, 李国庆*.拟康宁木霉T 51几丁质酶活性及内切几丁质酶基因克隆与分析[J].植物保护,2017,43(4):21. |
| 拟康宁木霉T 51几丁质酶活性及内切几丁质酶基因克隆与分析 |
| Chitinase activity and endo chitinase gene cloning and sequence analysis of Trichoderma koningiopsis T 51 |
| 投稿时间：2016-08-17 修订日期：2016-12-19 |
| DOI： |
| 中文关键词: 几丁质酶 拟康宁木霉 基因克隆 序列分析 |
| 英文关键词:chitinase Trichoderma koningiopsis gene cloning sequence analysis |
| 基金项目:公益性行业(农业)科研专项(201303025) |
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| 中文摘要: |
| 木霉是一类重要的生防真菌, 木霉产生的几丁质酶在其生物防治的重寄生过程中起着重要作用。拟康宁木霉Trichoderma koningiopsis T 51是一株对番茄灰霉病有生防潜力的木霉菌株, 本文测定了T 51菌株产生几丁质酶的活性, 结果表明T 51在PDB中液体培养以及与灰霉病菌在PDA上对峙培养时产生的几丁质酶活性显著受到灰霉病菌的诱导。采用RACE技术首次克隆了拟康宁木霉T 51中一个几丁质酶基因Tkchit42, 全长为1 817 bp, 包含4个外显子和3个内含子。预测该基因有一个1 275 bp的开放阅读框, 编码424个氨基酸, 预测的蛋白总分子量为46.378 kDa, 预测的等电点(pI)为5.16, 与T.koningii中42 kDa内切几丁质酶的氨基酸序列相似性达到99%。 |
| 英文摘要: |
| Trichoderma koningiopsis T 51 is a potential biological control agent against Botrytis cinerea. In this study, chitinase activity of T 51 was significantly induced by B. cinerea in both liquid PDB medium and the interacting area with B.cinerea colony on PDA medium. An endo chitinase gene Tkchit42 was cloned from T.koningiopsis T 51. The full length of Tkchit42 was 1 817 bp, encoding an endo chitinase protein containing 424 amino acid residues. The predicted protein molecular weight was 46.378 kDa. The Tkchit42 amino acid sequence BLAST showed 99% similarity with that of a 42 kDa endo chitinase of T. koningii. For our knowledge, this is the first report of chitinase gene cloned from T. koningiopsis |
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