| 张 宇1, 许萍萍2, 吴 晶2, 杨 静1, 李 彬1, 吴翠萍1*.进境高粱种子中葡萄茎枯病菌的检疫鉴定[J].植物保护,2017,43(3):192-198. |
| 进境高粱种子中葡萄茎枯病菌的检疫鉴定 |
| Identification of Didymella glomerata on imported Sorghum bicolor |
| 投稿时间：2016-08-01 修订日期：2016-09-27 |
| DOI： |
| 中文关键词: 葡萄茎枯病菌 形态特征 系统发育分析 致病性测试 |
| 英文关键词:Didymella glomerata morphological characters phylogenetic analysis pathogenicity test |
| 基金项目:国家质量监督检验检疫总局科技计划(2012IK278);“十二五”国家科技支撑计划(2012BAK11B03) |
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| 中文摘要: |
| 从进境的美国高粱样品中分离到一株与葡萄茎枯病菌Didymella glomerata相似的菌株4358-17。其菌落形态和显微特征均与葡萄茎枯病菌一致。多个位点(LSU、ITS、TUB2、ACT)序列比对显示菌株4358-17与GenBank登录号为KT389718、FJ427004、FJ427115、FJ426896的葡萄茎枯病菌相似性均达到100%。系统发育分析结果表明菌株4358-17与葡萄茎枯病菌株聚集在同一个分支上,支持率为99%。菌株4358-17接种高粱和小麦叶片,4 d后接种部位出现明显症状。根据上述试验结果,将进境美国高粱样品中分离的菌株4358-17鉴定为葡萄茎枯病菌。 |
| 英文摘要: |
| The isolate 4358-17 was obtained from imported Sorghum bicolor from America. It was similar to Didymella glomerata in morphology. BLAST analysis indicated that LSU, ITS, TUB2 and ACT sequence showed 100% identity with the sequences with accession numbers of KT389718, FJ427004, FJ427115 and FJ426896, respectively. Phylogenetic analysis revealed that the isolate 4358-17 clustered within D.glomerata strains. Pathogenicity test showed that it could cause leaf spot on sorghum and wheat 4 d after inoculation. Based on all above results, the isolate 4358-17 was identified as D.glomerata. |
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