| 马红霞, 张海剑, 孙 华, 石 洁*, 陈 丹, 郭 宁.玉米茎腐病病原菌检测方法研究[J].植物保护,2017,43(3):149-153. |
| 玉米茎腐病病原菌检测方法研究 |
| Comparison of pathogen detection methods for corn stalk rot |
| 投稿时间：2016-05-24 修订日期：2016-07-11 |
| DOI： |
| 中文关键词: 玉米茎腐病 镰孢菌 腐霉 |
| 英文关键词:corn stalk rot Fusarium spp. Pythium spp. |
| 基金项目:国家现代农业产业技术体系(CARS02) |
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| 中文摘要: |
| 为提高玉米茎腐病病原菌检测结果的准确性和可信度,以组织分离法做对比,采用分子检测法,对采自田间的189个玉米茎腐病病株进行真菌种类的鉴定和数量的统计。结果表明,分子检测法对腐霉Pythium spp.的检出频率为29.24%,对镰孢菌Fusarium spp.的检出频率为73.68%,组织分离法对腐霉的检出频率仅为0.58%,对镰孢菌的检出频率为60.82%,两方法的符合率最高仅为35.92%,最低为0。因此,采用组织分离和分子检测相结合的方法可提高玉米茎腐病病原菌鉴定结果的准确性。 |
| 英文摘要: |
| In order to improve the accuracy and credibility of detection results of pathogen causing corn stalk rot, the species of 189 fungal strains collected from the diseased corn plants in the field were identified and the number of fungi was counted by molecular detection and tissue isolation. The results showed that the detection frequency was 29.24% for Pythium spp. and 73.68% for Fusarium spp. by molecular detection, while the detection frequency was 0.58% for Pythium spp. and 60.82% for Fusarium spp. by tissue isolation. The highest coincidence rate was only 35.92% and the lowest was 0 between the two methods. Therefore, combination of tissue isolation and molecular detection methods can improve the accuracy of identification of the pathogen causing corn stem rot. |
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