| 杨世安1,2#, 李战彪2#, 秦碧霞2, 谢慧婷2, 崔丽贤2, 苏 琴2, 邓铁军2, 蔡健和2*.广西三种甜瓜病毒分离物的分子检测与鉴定[J].植物保护,2017,43(3):83-89. |
| 广西三种甜瓜病毒分离物的分子检测与鉴定 |
| Molecular detection and identification of three viruses isolated from melons in Guangxi |
| 投稿时间：2016-07-07 修订日期：2016-11-08 |
| DOI： |
| 中文关键词: 广西 瓜类褪绿黄化病毒 甜瓜黄化斑点病毒 甜瓜坏死斑点病毒 一步法RTPCR |
| 英文关键词:Guangxi Cucurbit chlorotic yellows virus (CCYV) Melon yellow spot virus(MYSV) Melon necrotic spot virus(MNSV) onestep RTPCR |
| 基金项目:国家现代农业产业技术体系广西特色水果创新团队项目(nycytxgxcxtd04192);广西自然科学基金(2015GXNSFBA139075);广西农业科学院科技发展基金(2015JZ48);河南郑州果树瓜类重点实验室开放基金(HNS20150810) |
| 作者 | 单位 | | 杨世安1,2#, 李战彪2#, 秦碧霞2, 谢慧婷2, 崔丽贤2, 苏 琴2, 邓铁军2, 蔡健和2* | 1. 广西大学农学院, 南宁 530004
2. 广西作物病虫害生物学重点实验室, 广西农业科学院植物保护研究所, 南宁 530007 |
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| 中文摘要: |
| 瓜类褪绿黄化病毒Cucurbits chlorotic yellows virus (CCYV)、甜瓜黄化斑点病毒Melon yellow spot virus (MYSV)及甜瓜坏死斑点病毒Melon necrotic spot virus (MNSV)是近年来报道侵染瓜类作物的新病毒,在个别种植区大面积发生,对生产构成严重威胁。为了解3种病毒在广西的发生情况,先后到各西甜瓜种植区进行了调查,并采集疑似CCYV、MYSV及MNSV的甜瓜病叶样品,提取病叶总RNA,通过特异性引物分别进行一步法RTPCR扩增,电泳结果显示RTPCR产物与预期大小一致的序列条带;将扩增产物分别连接到pMD19-T克隆载体上,挑选阳性克隆子进行序列测定及比对分析。结果表明:CCYV、MYSV和MNSV广西分离物的CP基因序列与其他已报道核苷酸序列一致性分别达95.1%~100%、96.5%~99%和83.7%~92.5%。 |
| 英文摘要: |
| Cucurbit chlorotic yellows virus (CCYV), Melon yellow spot virus (MYSV) and Melon necrotic spot virus (MNSV), which were emerging threat to melon plantation, have recently been found infecting melons in some large melongrowing areas. In order to understand the incidences of these three viruses, a survey was conducted on these viruses on melons, and leaves infected by CCYV, MYSV and MNSV were collected from Nanning and Beihai, Guangxi. Total RNAs were extracted from these diseased samples and RTPCR was performed by using specific primers. The PCR products were purified and inserted into pMD19-T cloning vector, and the expected DNA fragment were sequenced and analyzed by BLASTn in GenBank. The results showed that nucleocapsid protein gene sequences of CCYV, MYSV and MNSV isolates from Guangxi had 95.1%-100%, 96.5%-99% and 83.7%-92.5% identities with those of the isolates from other parts of China or some countries reported previously. |
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