| 袁莲莲1, 王耀锋2, 刘相甫3, 靳志伟4, 张 静5, 李 伟5, 董石飞5, 赵存孝2, 黄明迪6, 王凤龙1, 杨金广1*.蒙氏假单胞菌3A菌株对烟草漂浮育苗中TMV的钝化效果[J].植物保护,2017,43(2):37-42. |
| 蒙氏假单胞菌3A菌株对烟草漂浮育苗中TMV的钝化效果 |
| Passivation effect of Pseudomonas monteilii 3A on Tobacco mosaic virus in tobacco float seedlings |
| 投稿时间：2016-03-24 修订日期：2016-06-07 |
| DOI： |
| 中文关键词: 烟草普通花叶病毒(TMV) 蒙氏假单胞菌3A菌株 漂浮育苗 |
| 英文关键词:Tobacco mosaic virus (TMV) Pseudomonas monteilii 3A float seedling |
| 基金项目:甘肃省烟草公司科技项目(2015-08) |
| 作者 | 单位 | | 袁莲莲1, 王耀锋2, 刘相甫3, 靳志伟4, 张 静5, 李 伟5, 董石飞5, 赵存孝2, 黄明迪6, 王凤龙1, 杨金广1* | 1. 中国农业科学院烟草研究所, 国家烟草行业烟草病虫害监测与综合治理重点开放实验室, 青岛 266101
2. 甘肃省烟草公司庆阳市公司, 庆阳 745000
3. 中国烟叶公司, 北京 100055
4. 甘肃省烟草公司, 兰州 741306
5. 红云红河烟草(集团)有限责任公司, 昆明 650231
6. 甘肃省烟草公司陇南市公司, 陇南 746000 |
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| 中文摘要: |
| 选用对烟草花叶病毒Tobacco mosaic virus (TMV)具有显著拮抗活性的蒙氏假单胞菌Pseudomonas monteilii 3A菌株对烟草漂浮育苗过程中被TMV污染的育苗棚、育苗盘、基质及剪叶机械等进行生物钝化, 并利用real-time RT-PCR对污染基质中TMV的含量进行了定量检测。结果显示, 200、400倍及600倍P.monteilii 3A菌悬液稀释液均可显著钝化病苗残留干叶及干根中的TMV; 剪叶机械经不同稀释倍数的P. monteilii 3A菌悬液处理后, 在一定程度上抑制了TMV的发生, 相对防效达51.42%~100%; 基质经不同稀释倍数的P. monteilii 3A菌悬液处理后, 其中TMV的含量也下降了72.73%~88.46%, 对TMV的相对防效达61.56%~87.46%。其中, 200倍P. monteilii 3A稀释液处理育苗棚、育苗盘20 min以上, 浸泡剪叶机械1 min以上对TMV钝化效果最好。 |
| 英文摘要: |
| Pseudomonas monteilii 3A strain was selected to evaluate the passivation effect on Tobacco mosaic virus (TMV) in tobacco seedlings by real-time RT-PCR, combined with the incidence and disease index of the virus disease. The results showed that P. monteilii 3A bacterial liquid with different concentration gradients (200, 400 and 600×) had significant passivation effects on TMV in the residual dry roots and leaves of the old seedling sheds and trays. Meanwhile, using the clipping tool treated with P. monteilii 3A bacterial liquid could reduce the incidence and disease index of the virus disease, and the relative control effect was 51.42%-100%. P.monteilii 3A bacterial liquid could reduce the amount of TMV in the matrix polluted with TMV through bio-passivation by 72.73%-88.46%, and the relative control effect on the virus disease was 61.56%-87.46%. The best dilution of P.monteilii 3A bacterial liquid was 1∶200, and the optimum contact time on old seedling sheds and trays was no less than 20 min. Meanwhile, the clipping tools should be treated more than 1 min. |
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