| 贺 振1, 2, 李文凤3, 张志想2, 李世访2*.甘蔗线条花叶病毒HC-Pro基因的分子变异分析[J].植物保护,2017,43(2):29-36. |
| 甘蔗线条花叶病毒HC-Pro基因的分子变异分析 |
| Molecular variation of HC-Pro gene of Sugarcane streak mosaic virus |
| 投稿时间：2016-03-30 修订日期：2016-04-23 |
| DOI： |
| 中文关键词: 甘蔗线条花叶病毒 HC-Pro基因 分子变异 |
| 英文关键词:Sugarcane streak mosaic virus HC-Pro molecular variation |
| 基金项目:植物病虫害生物学国家重点实验室开放课题(SKLOF201518); 扬州大学科技创新培育基金(2015CXJ043) |
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| 中文摘要: |
| 为了解析甘蔗线条花叶病毒Sugarcane streak mosaic virus (SCSMV)不同分离物HC-Pro基因的分子变异规律, 本研究利用RT-PCR法扩增获得SCSMV HC-Pro基因的序列, 通过生物信息学分析, 分别从重组、系统发生、选择压力等方面研究SCSMV HC-Pro基因的分子变异特征。共测定了44条SCSMV HC-Pro基因序列, 相似性最低值为70%; HC-Pro基因重组频率较低, 仅发现3个重组位点, 其中一个系首次报道; 与先前报道相比, 部分新测定云南蔗区的SCSMV分离物在HC-Pro基因上形成一个新组-第Ⅲ组; HC-Pro基因处于很强的负选择压力作用, 未发现正向选择作用位点。本研究结果进一步证明SCSMV HC-Pro基因具有高度的遗传多样性。 |
| 英文摘要: |
| The objective of this study is to assess the molecular evolution and divergence of SCSMV according to HC-Pro gene sequences. The HC-Pro gene sequences of SCSMV were obtained by RT-PCR, and analyzed by bioinformatic software, in aspect of recombination, phylogenetics, selection, demography, and gene flow. In the present study, 44 HC-Pro gene sequences were determined with a 70% lowest similarity; only one novel recombination site together with two previous reported sites were found in HC-Pro gene, suggesting that infrequent recombination events were distributed in this gene of SCSMV; one novel lineage (lineage Ⅲ) clustered by SCSMV sequences determined here was found; strong purifying selection was found in the HC-Pro gene of SCSMV. Our genetic study further indicates that the HC-Pro gene showed high genetic diversity in the genome of SCSMV. |
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