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冯 皓, 祝一鸣, 王 伟, 刘 易, 贺 佳, 王 军, 单体江*.黄金香柳挥发油化学成分分析及其抗细菌活性[J].植物保护,2017,43(1):189-192.
黄金香柳挥发油化学成分分析及其抗细菌活性
Chemical composition and antibacterial activity of the volatile oils from Melaleuca bracteata
投稿时间:2016-01-06  修订日期:2016-02-21
DOI:
中文关键词:  黄金香柳  挥发油  化学成分分析  抗细菌活性
英文关键词:Melaleuca bracteata  volatile oil  chemical constituent analysis  antibacterial activity
基金项目:广东省林业科技创新项目(2015KJCH043); 广东省普通高校青年创新人才项目(2014KQNCX034)
作者单位
冯 皓, 祝一鸣, 王 伟, 刘 易, 贺 佳, 王 军, 单体江* 华南农业大学林学与风景园林学院, 广东省森林植物种质创新与利用重点实验室, 广州 510642 
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中文摘要:
      采用水蒸气蒸馏法提取黄金香柳枝叶中的挥发油, 得率为0.972% (以鲜重为基础)。通过GC-MS分析, 从黄金香柳枝叶中共鉴定出18种成分, 占总相对含量的99.056%, 其中主要成分是甲基丁香酚(92.185%)和肉桂酸甲酯(3.795%)。黄金香柳枝叶挥发油对6种供试细菌表现出一定的抑菌作用, 其中对黄瓜角斑病菌和根癌农杆菌的抑菌活性最好, 抑菌圈直径分别为(9.0±0.0)mm和(8.5±0.1)mm, 但仍弱于阳性对照硫酸链霉素对黄瓜角斑病菌(18.0±1.0)mm和根癌农杆菌(21.7±1.0)mm的活性。
英文摘要:
      Using hydro-distillation method, the volatile oil was extracted with a yield of 0.972% from fresh branches and leaves of Melaleuca bracteata. The chemical composition of the volatile oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Totally eighteen components were identified from the volatile oil, which accounted for 99.056% of total volatile substances. Methyl eugenol (92.185%) and methyl cinnamate (3.795%) were the major compounds of the volatile oil. The essential oil showed a certain antibacterial activity on all six tested bacteria, and had better inhibition to Pseudomonas lachrymans and Agrobacterium tumefaciens, with the diameters of inhibition zone of (9.0±0.0) mm and (8.5±0.1) mm, respectively, which was weaker than that of streptomycin sulfate. The diameters of inhibition zone treated with streptomycin sulfate was (18.0±1.0) mm and (21.7±1.0) mm, respectively.
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