| 王丽丽1, 周旭东2, 李国安1, 姚红燕1, 汪 峰1.番茄青枯病病原菌拮抗菌株的筛选及其田间防控作用研究[J].植物保护,2017,43(1):182-185. |
| 番茄青枯病病原菌拮抗菌株的筛选及其田间防控作用研究 |
| Screening and identifying of antagonistic bacteria against Ralstonia solanacearum and the control effects on tomato bacterial wilt in the field |
| 投稿时间：2016-01-28 修订日期：2016-03-23 |
| DOI： |
| 中文关键词: 番茄青枯病 拮抗菌 抗生素合成基因 |
| 英文关键词:tomato bacterial wilt antagonist bacteria antibiotic biosynthesis genes |
| 基金项目:宁波市科技局一般攻关项目(2014C10051) |
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| 中文摘要: |
| 从番茄青枯病发病严重田块的健康植株根际土壤中分离筛选得到2株高效拮抗菌株, 命名为W12和W118, 经16S rDNA基因鉴定均属芽胞杆菌属; 用PCR扩增的方法扩增脂肽类抗生素合成基因, 结果表明W12和W118含有合成bacillomycin、iturin和fengycin三种抗生素的基因; 将2株拮抗菌用于田间试验, 结果表明混合菌株防控效果最好, 3次灌菌后防控效果达到62.3%, 单独施用菌株W118较单独施用W12防控效果好, 3次灌菌后防控效果达到56.7%。 |
| 英文摘要: |
| Bacterial strains W12 and W118, isolated from the rhizosphere soil of tomato in Ningbo，could antagonize against Ralstonia solanacearum. Based on the 16S rDNA sequence analysis, W12 and W118 were preliminarily identified as Bacillus spp. The genes responsible for biosynthesis of bacillomycin, iturin and fengycin were identified in both W12 and W118 strains by PCR amplification. The field trials showed that the control effect of mixed bacterial suspension of W12 and W118 reached 62.3% after irrigations for three times, while when used alone, the control effect of strain W118 was 56.7%, which was better than that of strain W12. |
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