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田彩红1, 封洪强1*, 王 瑛1, 许 鹏1, 2, 李国平1, 黄建荣1, 封洪云1, 尹新明2.中黑盲蝽胚胎发育的显微及切片观察[J].植物保护,2017,43(1):178-181.
中黑盲蝽胚胎发育的显微及切片观察
Morphological observation of embryonic development of Adelphocoris suturalis by microscope and paraffin section
投稿时间:2016-02-06  修订日期:2016-03-16
DOI:
中文关键词:  中黑盲蝽  胚胎发育  滞育卵  石蜡切片  卵重
英文关键词:Adelphocoris suturalis  embryo development  diapause eggs  paraffin section  weight of eggs
基金项目:公益性行业(农业)科研专项(201103012-7); 国家自然科学基金(31101449)
作者单位
田彩红1, 封洪强1*, 王 瑛1, 许 鹏1, 2, 李国平1, 黄建荣1, 封洪云1, 尹新明2 1. 河南省农作物病虫害防治重点实验室, 农业部华北南部作物有害生物综合治理重点实验室, 河南省作物保护国际联合实验室, 河南省生物农药工程研究中心, 河南省农业科学院植物保护研究所, 郑州 450002
2. 河南农业大学植物保护学院, 郑州 450002 
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中文摘要:
      以滞育卵越冬是中黑盲蝽生活史的重要组成部分, 为明确滞育卵胚胎发育的停滞时期, 对中黑盲蝽的胚胎发育过程进行了显微观察, 并将胚胎发育停滞期的滞育卵做了石蜡切片。温度(26±1)℃, 相对湿度70%±10%, 光周期L∥D=14 h∥10 h条件下, 滞育卵在产卵后48 h停止发育, 而非滞育卵则继续发育, 在产卵后第9天孵出若虫。通过测定中黑盲蝽胚胎发育不同时期的卵重发现, 非滞育卵随着胚胎发育卵重持续增加, 滞育卵在发育48 h后卵重不再增加。
英文摘要:
      Adelphocoris suturalis overwinters with diapause eggs laid in the stems of cotton, tissue of weed, fruit trees and alfalfa hosts adjacent to the cotton fields, which is the most critical link of its life history. To identify the period of the embryo development stagnation in diapause eggs, we performed a morphological observation using microscope and paraffin section. Eggs, nymphs and adults were reared on green beans at (26±1)℃, 70%±10% relative humidity, and a photoperiod cycle of 14 h light/10 h dark. The embryo stopped growing within 48 hours after oviposition in diapause eggs. While in the non-diapause eggs, the embryo kept developing with cells differentiation, 9 days after oviposition, the nymphs were hatched. The weight of non-diapause eggs continued to increase with the development of embryo. However, the weight of diapause eggs maintained stability after the formation of diapause.
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