| 范 昆1, 付 丽1, 李晓军1, 翟 浩1, 刘 璐2, 曲健禄1*.苹果轮纹病菌对戊唑醇的敏感性及其抗性突变体的致病力[J].植物保护,2017,43(1):140-147. |
| 苹果轮纹病菌对戊唑醇的敏感性及其抗性突变体的致病力 |
| Susceptibility of Botryosphaeria dothidea to tebuconazole and virulence of its resistant mutants |
| 投稿时间：2016-02-16 修订日期：2016-04-26 |
| DOI： |
| 中文关键词: 苹果轮纹病菌 戊唑醇 敏感性 突变体 致病力 |
| 英文关键词:Botryosphaeria dothidea tebuconazole susceptibility mutant pathogenicity |
| 基金项目:山东省自然科学基金(ZR2013CQ040); 国家现代农业产业技术体系(CARS-28); 泰安市科技攻关项目(201540699); 山东省农业科学院农业科技创新工程（CXGC2016B07）; 山东省农业科学院青年科研基金(2016YQN32); 山东省果树研究所所长基金(2013ky02) |
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| 中文摘要: |
| 为明确苹果轮纹病菌对戊唑醇的敏感性及其抗性突变体的致病力, 采用菌丝生长速率法测定了130株苹果轮纹病菌菌株对戊唑醇的敏感性, 采用药剂驯化和紫外线诱导的方式获得抗性突变体, 并研究其生存适合度。结果表明, 戊唑醇对130个菌株的EC50分布在0.051 6~1.925 4 μg/mL, 均值为(0.799 6±0.039 1)μg/mL, 表明苹果轮纹病菌开始出现敏感性下降的亚群体。供试苹果轮纹病菌菌株的抗性频率为71.54%, 各省份均未出现高抗菌株。药剂驯化、紫外诱导共获得3株抗性倍数分别为58.82倍、41.60倍、38.19倍的高抗菌株。抗性突变体致病力、适合度指数与敏感菌株相比没有显著性差异。无药培养9代后, 各抗性突变体EC50逐渐下降, 所获抗性可能不能稳定遗传。表明苹果轮纹病菌对戊唑醇的抗性在田间自然选择压力下可能会逐渐降低。 |
| 英文摘要: |
| 130 Botryosphaeria dothidea isolates were collected from major apple-produced regions to assess the susceptibility to tebuconazole by mycelium growth rate method and to test the virulence of resistance mutants. Resistant mutants were obtained by chemical screening and UV irradiation in the laboratory, and the fitness parameters of resistant and susceptible isolates were measured. The results showed that the EC50 values of 130 isolates varied from 0.051 6-1.925 4 μg/mL with the mean of (0.799 6±0.039 1)μg/mL and low resistant subcolony has been discovered. The resistant ratio of tested isolates was 71.54% and high-resistant strains has not discovered in major apple-produced regions. The resistance level of three resistant mutants was high with the resistant ratio of 58.82, 41.60 and 38.19 times, respectively. The fitness and virulence of resistant mutants had no significant difference between resistant and susceptible isolates. The resistance ratio of mutants decreased gradually after 9 generations of subcultures in the absence of fungicides and the resistance could not inherit stably. The results indicated that the resistance of B. dothidea to tebuconazole could gradually decline when the field selection pressure became lower. |
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