| 由书妍1, 刘红霞1, 于瑞君1, 郑丽君1, 范诗言1, 李红叶2*.柑橘绿霉病菌木聚糖酶基因(PdXY2)功能初步研究[J].植物保护,2016,42(6):95-99. |
| 柑橘绿霉病菌木聚糖酶基因(PdXY2)功能初步研究 |
| Preliminary study on the function of PdXY2 in Penicillium digitatum |
| 投稿时间：2015-12-24 修订日期：2016-02-24 |
| DOI： |
| 中文关键词: 柑橘绿霉病菌 木聚糖酶 基因表达 基因突变 |
| 英文关键词:Penicillium digitatum Xylanase gene expression gene disruption |
| 基金项目:国家自然科学基金(31371961); 国家现代农业产业技术体系(CARS27) |
|
| 摘要点击次数: 802 |
| 全文下载次数: 884 |
| 中文摘要: |
| 柑橘绿霉病菌(Penicillium digitatum)是引起柑橘储藏期腐烂最主要的病原之一, 严重影响了柑橘产业发展。本试验克隆了柑橘绿霉病菌的木聚糖酶基因(PdXY2), 对其表达进行研究, 在柑橘发病过程中, PdXY2的表达显著升高, 至48 h达到最高, 其表达量为对照的4倍。为进一步明确PdXY2基因功能, 构建了PdXY2基因缺失突变株(ΔPdXY2), ΔPdXY2突变株的致病性与野生型相比没有明显差异。由此我们推断, 在柑橘绿霉病菌侵染柑橘的过程中PdXY2有一定的作用, 但是单一缺失木聚糖酶PdXY2基因不能影响其致病性。 |
| 英文摘要: |
| Penicillium digitatum is one of the most important pathogen causing green mold disease which impedes the development of citrus industry. In this study, we cloned a xylanase gene PdXY2 and characterized its functions via knock-out strategy. The expression of PdXY2 was up-regulated during the primary stage of infection, and reached a peak at 48 h. However, PdXY2-disrupted mutant (ΔPdXY2) showed no reduction in virulence. Taken together, our results demonstrated that PdXY2 may play an important role in infection and single knock-out of PdXY2 showed no reduction in virulence. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
