| 满益龙1,2, 谭新球2*, 司乃国3, 陈 岳2, 张 卓2, 刘 勇1,2, 张德咏1,2*.不同辣椒炭疽病菌对唑菌酯的敏感性差异[J].植物保护,2016,42(5):171-176. |
| 不同辣椒炭疽病菌对唑菌酯的敏感性差异 |
| Sensitivity differentiation of different Colletotrichum spp. strains causing anthracnose of Capsicum annuum L.plants to pyraoxystrobin |
| 投稿时间：2016-03-06 修订日期：2016-07-06 |
| DOI： |
| 中文关键词: 辣椒炭疽病菌 唑菌酯 生物测定 敏感性 |
| 英文关键词:Colletotrichum spp. pyraoxystrobin bioassay sensitivity |
| 基金项目:国家自然科学基金（31471831）; “十二五”国家科技支撑计划（2014BAD05B04-4） |
| 作者 | 单位 | | 满益龙1,2, 谭新球2*, 司乃国3, 陈 岳2, 张 卓2, 刘 勇1,2, 张德咏1,2* | 1. 湖南农业大学植物保护学院, 长沙 410128
2. 园艺作物病虫害治理湖南省重点实验室, 湖南省农业科学院植物保护研究所, 长沙 410125
3. 沈阳化工研究院, 沈阳 110021 |
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| 中文摘要: |
| 辣椒炭疽病是辣椒生产上的重要病害，严重制约辣椒生产，唑菌酯防治辣椒炭疽病的相关研究未见报道。为此，本研究利用菌丝生长速率法测定了4种不同炭疽病菌对唑菌酯的敏感性，并对其作用靶标基因细胞色素b基因(Cytb)进行比较分析。结果表明:唑菌酯对Colletotrichum brevisporum YYGXZ07，C.truncatum CZHP03，C.acutatum HHBY48和C.gloeosporioides CSLL11的EC50分别为0.098、3.363、10.156和31.982 μg/mL，而且供试菌株在含药培养基上的生长速率排序为C. truncatum CZHP03 |
| 英文摘要: |
| The anthracnose disease caused by Colletotrichum spp. is an important limited factor in pepper production in the world. Many chemicals have been used to control this disease in the fields. However, so far, pyraoxystrobin, a new strobilurin fungicide, hasn’t been reported in the disease control. Therefore, sensitivities of four different Colletotrichum strains to pyraoxystrobin were determined through bioassay of mycelial growth rate, and target gene of pyraoxystrobin, cytochrome b (Cyt b) of different Colletotrichum species was cloned and analyzed in this study. The results showed that the EC50value of pyraoxystrobin to C.brevisporum YYGXZ07,C.truncatum CZHP03,C.acutatum HHBY48 and C.gloeosporioides CSLL11 was 0.098,3.363,10.156 and 31.982 μg/mL,respectively. The mycelial growth rate was ranked as C.truncatum CZHP03< C.brevisporum YYGXZ07 < C.acutatum HHBY48< C.gloeosporioides CSLL11. BLAST analysis indicated that the similarity of amino acid sequences of cytochrome b of C.acutatum HHBY48 with C.truncatum CZHP03, C.brevisporum YYGXZ07 and C.gloeosporioides CSLL11 was 82.98%, 88.30% and 87.77% respectively. From the alignment of the amino acids sequences, we preliminary inferred that the sensitivity differentiation of the four Colletotrichum species may cause by the differences of six amino acids in the cytochrome b sequences |
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