| 杨腊英, 郭立佳, 汪 军, 刘 磊, 梁昌聪, 王国芬, 陈平亚, 黄俊生*.香蕉枯萎病菌SIX2基因序列及特异性分析[J].植物保护,2016,42(5):118-124. |
| 香蕉枯萎病菌SIX2基因序列及特异性分析 |
| Sequence and specificity analysis of SIX2 gene of Fusarium oxysporum f.sp. cubense |
| 投稿时间：2015-09-29 修订日期：2015-12-18 |
| DOI： |
| 中文关键词: 尖孢镰刀菌古巴专化型 SIX2 寄主选择性 分子检测 |
| 英文关键词:Fusarium oxysporum f.sp.cubense SIX2 host selection molecular diagnosis |
| 基金项目:国家自然科学基金（31301629） |
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| 中文摘要: |
| 依据侵染的香蕉品种范围不同，引起香蕉枯萎病的尖孢镰刀菌古巴专化型(Fusarium oxysporum f.sp. cubense，Foc)分为4个生理小种。Foc在寄主植物木质部分泌的SIX (secreted in xylem)蛋白可能与不同生理小种侵染的香蕉品种范围不同存在密切关系，找到 Foc 4号生理小种(Foc4)特有的SIX蛋白编码基因将有利于进一步分析Foc4寄主范围更广的原因，从而开展抗病育种工作。采用PCR方法比较分析了国内不同地理区域及来源于澳大利亚与南非的Foc1、Foc2、Foc3、Foc4共56株菌株中的SIX2基因，并以8个以上其他专化型或其他种或属的热带作物病原菌共39株菌株分析了Foc4 SIX2基因序列的特异性，分析了SIX2基因的灵敏度及利用其检测感病植株。仅从供试的Foc4菌株基因组DNA中扩增出SIX2基因序列，检测的DNA灵敏度达5 pg/25 μL，并可用于检测感病的球茎组织。Foc4中特有的SIX2基因序列为特异性鉴定感病植株病原菌种类提供了快速分子检测技术，为明确该基因是否决定性影响Foc4对寄主差异性的选择研究提供了基础。 |
| 英文摘要: |
| Fusarium oxysporum f.sp. cubense (Foc), causal agent of Fusarium wilt of banana, has been divided into four races according to cultivar specificity. There may be a close relationship between the races infested different banana varieties and the proteins secreted in xylem (SIX) by Foc when it invaded into the host plant xylem. The specific SIX gene will be conductive to further analyzing the reasons for broader host range of Foc4 and helpful for the disease-resistant cultivar breeding. Using PCR and sequencing approach, SIX2 gene were analyzed in 56 Foc isolates collected from different geographic areas of Southern China, Australia and South Africa. The specificity and sensitivity of the primers were determined by using other thirty-nine isolates including eight other F.oxysporum formae specialis, two non-pathogen F. oxysporum and other genus or species of some tropical plant pathogens. With the designed primers, the target band was detected only in the DNA of Foc4 isolates, and the sensitivity of the primers were 5 pg/25 μL and could be used to detect diseased bulb section. Therefore, SIX2 gene specific in Foc4 could be applied for rapid molecular identification of the pathogens in susceptible plants, which will provide a basis for further research on whether the gene is clearly a decisive influence on the host selection of Foc4. |
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