| 吴 彦1, 尤春雪2, 田兆福1, 郭姗姗2, 刘绍华1, 黄东业1, 邓宾玲1, 李志华1, 白家峰1, 杜树山2*.牡荆叶挥发油对烟草甲的杀虫活性[J].植物保护,2016,42(5):97-102. |
| 牡荆叶挥发油对烟草甲的杀虫活性 |
| Chemical composition and insecticidal activities of Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz. essential oil against the cigarette beetle |
| 投稿时间：2015-10-20 修订日期：2015-11-27 |
| DOI： |
| 中文关键词: 牡荆叶 烟草甲 触杀活性 熏蒸活性 |
| 英文关键词:Vitex negundo var. cannabifolia Lasioderma serricorne contact toxicity fumigant toxicity |
| 基金项目:广西科学研究与技术开发计划课题（桂科攻15118004）；北京市自然科学基金(7142093) |
| 作者 | 单位 | | 吴 彦1, 尤春雪2, 田兆福1, 郭姗姗2, 刘绍华1, 黄东业1, 邓宾玲1, 李志华1, 白家峰1, 杜树山2* | 1. 广西中烟工业有限责任公司技术中心, 南宁 530001
2. 北京师范大学中药资源保护与利用北京市重点实验室, 北京 100875 |
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| 中文摘要: |
| 用水蒸气蒸馏法提取牡荆叶挥发油，并采用气相色谱-质谱联用法分析其化学成分，基于柱色谱和核磁共振方法分离并鉴定化合物。从牡荆叶挥发油中鉴定出47种化合物，主要成分为β-石竹烯(25.8%)、桉油精(9.5%)、石竹烯氧化物(7.3%)和乙酸松油酯(6.1%)。并对牡荆叶挥发油及其分离得到的β-石竹烯和桉油精进行了杀虫活性测定。结果表明牡荆叶挥发油对烟草甲(Lasioderma serricorne)成虫具有触杀毒性(LD50为25.30 μg/头)和熏蒸毒性(LC50为13.12 mg/L。挥发油中的主要化合物β-石竹烯和桉油精对烟草甲均有一定的触杀毒性(LD50分别为41.75 μg/头和15.58 μg/头)。桉油精对烟草甲还显示出较强的熏蒸活性(LC50为5.18 mg/L)。结果提示牡荆叶挥发油及其活性化合物可用于烟草甲的综合防治。 |
| 英文摘要: |
| In order to find and develop new botanical pesticides against tobacco storage pests, bioactivity screening was performed. The essential oil obtained from the leaves of Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz. was investigated by GC-MS.A total of 47 components of the oil were identified and the main components in the oil were found to be β-caryophyllene (25.8%) and eucalyptol (9.5%), followed by caryophyllene oxide (7.3%) and terpinyl acetate (6.1%). After screening, the essential oil exhibited potential insecticidal activity. In the process of assay, the essential oil was found to possess strong contact toxicity against Lasioderma serricorne adults with a LD50 value of 25.30 μg/adult and fumigant toxicity with a LC50 value of 13.12 mg/L air. β-caryophyllene and eucalyptol also possessed strong contact toxicity against L.serricorne with a LD50 value of 41.75 and 15.58 μg/adult, respectively. Moreover, eucalyptol exhibited strong fumigant toxicity against the cigarette beetle with a LC50 value of 5.18 mg/L air. The results indicate that the essential oil of Vitex negundo L.and the two isolated compounds has potential to be developed into a natural insecticide/fumigant for control of the cigarette beetle |
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