| 刘聪利, 李明*, 赵改荣, 李玉红.河南甜樱桃病毒病害调查及病原检测[J].植物保护,2016,42(4):200-204. |
| 河南甜樱桃病毒病害调查及病原检测 |
| Investigation and pathogen detection of sweet cherry virus disease in Henan |
| 投稿时间：2015-08-24 修订日期：2015-10-10 |
| DOI： |
| 中文关键词: 甜樱桃 病毒病 病原检测 复合侵染 |
| 英文关键词:sweet cherry virus disease pathogen detection mixed infection |
| 基金项目:中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2015-ZFRI)；郑州市现代农业科技创新工程（131PZDGC113） |
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| 中文摘要: |
| 在河南省郑州市、巩义市、荥阳市、新郑市选择具有代表性的甜樱桃生产园对病毒病发生情况进行调查, 采集表现为疑似病毒病症状的样本65份, 利用7种病毒引物进行RT-PCR检测。5种病毒检测结果呈阳性, 分别是李属坏死环斑病毒(Prunus necrotic ringspot virus, PNRSV)、李矮缩病毒(Prune dwarf virus, PDV)、樱桃绿环斑驳病毒(Cherry green ring mottle virus, CGRMV)、樱桃坏死锈斑病毒(Cherry necrotic rusty mottle virus, CNRMV)及樱桃病毒A(Cherry virus A, CVA); 序列分析结果表明, 5种病毒扩增片段与GenBank中注册的相应病毒核苷酸序列均具有较高 的一致性; 样本病毒检出率为100%, 其中13份样本为单独侵染, 其余52份样本均为多病毒复合侵染, 占比高达80%, 复合侵染比例随着侵染病毒种类的增多逐渐降低; 病毒侵染组合与叶片表型症状无明显对应关系。 |
| 英文摘要: |
| Several representative sweet cherry orchards were selected to investigate the occurrence condition of virus disease in Zhengzhou, Gongyi, Xingyang and Xinzheng cities of Henan Province, from which sixty-five samples with suspected virus symptoms were collected. Seven pairs of specific primers were used to detect viruses infected sweet cherry by RT-PCR. RT-PCR results and sequence analysis showed that five viruses were found positive, including Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Cherry green ring mottle virus (CGRMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry virus A (CVA). The amplified fragments had high identity with the corresponding nucleotide sequence reported in GenBank. The positive rate of viruses was 100%, among all 65 samples tested, 13 were of single infection, and the rest 52 samples were of mixed infection which account for more than 80%.The proportion of mixed infection gradually decreased along with ascending of the virus types. No significantly comparable relations were found between types of mixed infection and virus disease symptoms. |
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