| 任毅1, 王修清1, 孟庆恒1, 孙建华1, 冯欣1*, 黄文坤2, 彭德良2.真菌Sr18代谢产物对根结线虫胁迫下黄瓜叶片保护酶的影响[J].植物保护,2016,42(4):99-104. |
| 真菌Sr18代谢产物对根结线虫胁迫下黄瓜叶片保护酶的影响 |
| The effects of Syncephalastrum racemosum Sr18 metabolites oncucumber protective enzymes under root-knot nematode stress |
| 投稿时间：2016-01-29 修订日期：2016-04-22 |
| DOI： |
| 中文关键词: Sr18丝状真菌 根结线虫 保护酶 抗性机理 |
| 英文关键词:Syncephalastrum racemosum root-knot nematode protective enzymes defence mechanism |
| 基金项目:国家自然科学基金（31272019, 31272022）；国家科技支撑计划（2012BAD19B06） |
| 作者 | 单位 | | 任毅1, 王修清1, 孟庆恒1, 孙建华1, 冯欣1*, 黄文坤2, 彭德良2 | 1. 天津市动植物抗性重点实验室, 天津师范大学生命科学学院, 天津300387
2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京100193 |
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| 中文摘要: |
| 为探究根结线虫胁迫下丝状真菌Sr18代谢产物对黄瓜的作用机理, 采用温室盆栽及人工接种试验, 研究了不同浓度的Sr18代谢产物对南方根结线虫胁迫下黄瓜叶片保护酶的影响。结果表明, 线虫侵染黄瓜根部以后, 黄瓜叶片SOD、POD和CAT活性减弱, PPO和PAL浓度降低。施加不同浓度的Sr18代谢产物, 能够使线虫胁迫下的黄瓜叶片SOD、POD和CAT活性增强, 使PPO和PAL的含量增加, 说明Sr18代谢产物能够提高黄瓜的保护酶活性与含量, 增强黄瓜对南方根结线虫的抗性。 |
| 英文摘要: |
| To understand the action mechanism of Syncephalastrum racemosum Sr18 metabolites to cucumber under the root-knot nematode Meloidogyne incognita stress, the effects of Sr18 metabolites on five protective enzymes (SOD, POD, CAT, PAL, PPO) of cucumbers with/without seedling inoculation by nematodes were investigated in greenhouses. The results showed that higher levels of SOD, POD, CAT activities and PPO, PAL contents were observed in the plants pretreated with Sr18 and inoculated with nematodes than that in non-treated control, while lower activities of SOD, POD and CAT and lower contents of PPO and PAL in leaves of cucumber were observed in the plants inoculated with nematodes than that in non-inoculated control. Sr18 metabolites induced the upregulated activity of antioxidative enzymes in cucumber leaves and enhanced the resistance level of cucumber against the root-knot nematode M. incognita. |
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