| 李登辉1, 2, 郭焕强1, 马丽3, 马东方2, 王凤涛1, 冯晶1, 蔺瑞明1*, 徐世昌1*.我国大麦条纹病菌(Pyrenophora graminea)群体遗传多样性AFLP分析[J].植物保护,2016,42(4):71-76. |
| 我国大麦条纹病菌(Pyrenophora graminea)群体遗传多样性AFLP分析 |
| Genetic diversity analysis of Pyrenophora graminea populations in China, the causal organism of barley leaf stripe by using AFLP method |
| 投稿时间：2015-07-27 修订日期：2015-10-15 |
| DOI： |
| 中文关键词: 大麦 条纹病 麦类核腔菌 AFLP标记 聚类分析 |
| 英文关键词:Hordeum vulgare leaf stripe Pyrenophora graminea AFLP marker cluster analysis |
| 基金项目:国家现代农业（大麦）产业技术体系（CARS-05）；公益性行业（农业）科研专项（201303016） |
| 作者 | 单位 | | 李登辉1, 2, 郭焕强1, 马丽3, 马东方2, 王凤涛1, 冯晶1, 蔺瑞明1*, 徐世昌1* | 1. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
2. 长江大学农学院, 荆州434025
3. 河南农业职业学院, 郑州 451450 |
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| 中文摘要: |
| 条纹病是重要的种传真菌病害之一, 能对大麦生产造成严重产量损失。本研究利用AFLP方法对来自青海、河南等10省份大麦种植区的条纹病菌菌株进行遗传多样性分析, 从分子水平上揭示我国不同大麦产区的条纹病菌群体遗传差异。结果显示, 利用8对AFLP选择性引物组合对19份大麦条纹病菌进行了全基因组多态性扩增, 共获得144条可统计的条带, 其中112条具有多态性, 多态性条带占77.8%。在相似系数为0.83时, 可将19份大麦条纹病菌菌株划分为4个类群, 多数菌株聚类在类群Ⅰ和类群Ⅱ中, 类群Ⅲ和类群Ⅳ中仅各包含1个菌株, 且与其他2个类群中的成员亲缘关系较远。因此, 我国大麦条纹病菌群体中存在一定程度的遗传变异。另外, 发现菌株间亲缘关系远近与其分布地域无明显规律。 |
| 英文摘要: |
| Leaf stripe is one of the most destructive seed-borne fungal diseases on barley, which usually causes great economic losses in barley production. In this study, in order to disclose genetic differences between the pathogen populations of Pyrenophora graminea derived from different barley-growing areas at molecular level, the fungal pathogen isolates were collected from the barley-growing areas in 10 provinces, such as Qinghai and Henan, etc., to investigate the genetic diversity among these pathogen populations by using AFLP method. The DNA genetic polymorphism of 19 barley stripe pathogen isolates were detected with 8 selective AFLP primer combinations at global genomic level, and totally 144 countable bands were produced, of which 112 bands (77.8% of the total) were polymorphic. At a similar coefficient of 0.83, the 19 isolates were clustered into 4 P. graminea groups (PGs). Most of them were included in PGⅠand PGⅡ, but there was only one member in PG Ⅲ and PG Ⅳ, respectively, with a longer distance for the members in the other two PGs. Therefore, there is some genetic diversity in the pathogen population in the barley-growing areas across China. Besides, it was found that there was no significant relationship between genetic similarity and collection locations of the pathogen isolates. |
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