| 贾娇, 苏前富, 孟玲敏, 张伟, 李红, 刘婉丽, 晋齐鸣*.抗咯菌腈禾谷镰刀菌的紫外诱导及其生物学特性[J].植物保护,2016,42(4):43-47. |
| 抗咯菌腈禾谷镰刀菌的紫外诱导及其生物学特性 |
| Ultraviolet induction and characteristics of Fusariumgraminearum resistant to fludioxonil |
| 投稿时间：2015-08-11 修订日期：2015-10-23 |
| DOI： |
| 中文关键词: 咯菌腈 禾谷镰刀菌 交互抗性 |
| 英文关键词:fludioxonil Fusarium graminearum cross-resistance |
| 基金项目:国家现代农业(玉米)产业技术体系(CARS-02); 吉林省科技厅青年科技基金(20140520158JH) |
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| 中文摘要: |
| 为评估玉米茎腐病病原菌禾谷镰刀菌(Fusarium graminearum)对咯菌腈的室内抗药性风险, 本研究通过室内紫外照射获得抗咯菌腈突变体, 分析抗性突变体对咯菌腈的抗药性、遗传稳定性和生物学特性, 及其对咯菌腈、戊唑醇、苯醚甲环唑、嘧菌酯的交互抗性。结果表明, 经紫外照射5 min, 获得16株抗咯菌腈突变体, 其对咯菌腈的EC50为72.78~290.09 μg/mL, 是亲本菌株的4 000~17 000倍; 抗性突变频率为1.7×10-6, 可稳定遗传; 最适生长温度均为25℃, 最适pH均为8, 与亲本菌株相同; 菌落生长速度低于亲本菌株; 在含有0.9 mmol/L NaCl的PDA培养基中培养的菌落形态与不含NaCl的PDA培养基中的相比, 亲本菌株和4号抗性菌株色素沉积减少, 而1号和17号抗性菌株色素沉积增加。推测禾谷镰刀菌对咯菌腈存在中等或高等的室内抗药性风险。室内抗药性测定表明抗性突变体对咯菌腈和苯醚甲环唑均产生了抗性。 |
| 英文摘要: |
| To assess the resistance risk of Fusarium graminearum to fludioxonil in the laboratory, 17 UV-induced mutants resistant to fludioxonil were obtained. The resistance, stability and biological characteristics of the mutants were studied. Cross-resistance of the mutants to fludioxonil, tebuconazole, difenoconazole and azoxystrobin was tested. The results showed that EC50 values of the mutants to fludioxonil ranged from 72.78 μg/mL to 290.09 μg/mL and the ratio of EC50values of mutants resistant to fludioxonil, compared with that of its parental strains, was 4 000-17 000 times. The frequency of resistance mutation was 1.7×10-6 and the resistance obtained was stable. The optimum temperature for the colony growth was 25℃, and the optimum pH value was 8 for both resistant isolates and their parental strains. The growth rates of resistant mutants were lower than those of their parental strains. The pigment of parental strains and resistant isolate 4 in PDA containing 0.9 mmol/L NaCl was lower than that in PDA without NaCl, but the pigment of resistant isolates 1 and 16 was higher. Fludioxonil-resistant isolates of F. graminearum showed moderate or high risk and have developed resistance to azoxystrobin. |
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