| 左琳彧#, 雍容婧#, 袁雯, 杜开通, 周涛*.北京地区辣椒上黄瓜花叶病毒和甜菜西方黄化病毒复合侵染的分子鉴定[J].植物保护,2016,42(3):181-183. |
| 北京地区辣椒上黄瓜花叶病毒和甜菜西方黄化病毒复合侵染的分子鉴定 |
| Molecular identification of co-infection of Cucumber mosaic virus and Beet western yellows virus on pepper in Beijing |
| 投稿时间：2015-05-23 修订日期：2015-07-23 |
| DOI： |
| 中文关键词: 蚜传病毒 黄瓜花叶病毒 甜菜西方黄化病毒 复合侵染 RT-PCR |
| 英文关键词:aphid-borne virus Cucumber mosaic virus Beet western yellows virus co-infection RT-PCR |
| 基金项目:公益性行业(农业)科研专项(201303028)；国家大学生科技创新项目 |
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| 中文摘要: |
| 辣椒是我国重要的蔬菜和经济作物, 受多种病毒危害。2014年在北京市顺义区调查时发现部分种植的辣椒植株上叶片大面积黄化, 边缘症状明显, 个别植株叶片轻微上卷。提取典型症状样品的总RNA, 反转录得到cDNA, 分别用黄瓜花叶病毒(Cucumber mosaic virus, CMV)特异引物和马铃薯卷叶病毒属(Polerovirus)通用引物进行PCR检测, CMV特异引物和马铃薯卷叶病毒属通用引物分别扩增得到约650 bp和1 400 bp的特异条带。测序和核苷酸序列比对表明, 其分别与CMV和甜菜西方黄化病毒(Beet western yellows virus, BWYV)序列同源性最高为99%和96%。这是对我国种植的辣椒上发生的CMV和BWYV复合侵染的首次报道。 |
| 英文摘要: |
| Pepper, an important vegetable and economic crop, is susceptible to many viruses. In a survey of virus diseases on vegetables in 2014, some pepper plants showing typical yellow leaf symptom were found in Shunyi District, Beijing, China. Total RNA was isolated from the sample with typical symptom, and reverse transcript-PCR assays were performed with specific primers for Cucumber mosaic virus (CMV) and degenerate primers for Polerovirus. Two specific DNA fragments, one about 650 bp and another 1 400 bp, were obtained using primers for CMV and Polerovirus, respectively. Nucleotide sequence analysis showed that they had the highest identity of 99% and 96% with that of CMV and Beet western yellows virus (BWYV), separately. To our knowledge, this is the first report of co-infection of CMV and BWYV on pepper plants. |
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