| 顾沁, 张昊, 黄海, 丁正, 伍辉军, 高学文*.一种玉米新型细菌性褐腐病的病原鉴定[J].植物保护,2016,42(3):87-90. |
| 一种玉米新型细菌性褐腐病的病原鉴定 |
| Identification of the bacteria causing a new brown stalk rot on maize |
| 投稿时间：2015-10-29 修订日期：2015-11-30 |
| DOI： |
| 中文关键词: 玉米病害 gyrB 基因 菠萝泛菌 分散泛菌 |
| 英文关键词:corn disease gyrB gene Pantoea dispersa Pantoea ananatis |
| 基金项目:公益性行业(农业)科研专项(20130315)；国家高技术研究发展计划(2012AA101106)；江苏省重点研发计划(现代农业)(BE2015354) |
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| 中文摘要: |
| 本研究从河北省武邑、献县、阜城和永年采集感病玉米植株。病株症状为叶梢枯死卷曲, 叶脉产生黄色病斑, 茎秆处黄褐色干腐。从发病植株中分离得到了10株疑似病原细菌菌株, 经过回接验证和16S rDNA和 gyrB 基因的序列分析, 结果表明, 引起该病害的病原物为菠萝泛菌(Pantoea ananatis)和分散泛菌(Pantoea dispersa)。这是国内首次报道菠萝泛菌和分散泛菌引起玉米细菌性病害。 |
| 英文摘要: |
| To identify the pathogens of newly occurred maize disease, we collected the samples of maize disease from four areas (Wuyi, Xianxian, Fucheng and Yongnian) in Hebei Province. The symptoms of the disease showed yellowish-brown dry rot in corn stalk, curl and blight symptoms on leaf tips and yellow spots on leaf veins. Pathogenic tests were assayed by inoculating the bacteria isolated from the diseased corn plants. The pathogens were identified as Pantoea ananatis and P. dispersa according to the sequence analysis of 16S rDNA and gyrB gene. To our knowledge, it is the first report of P. ananatis and P. dispersa causing maize disease in China. |
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