| 龚莉君1, 彭鹏1, 袁哲明1, 2, 柳傲1, 杨中侠1, 2*.小菜蛾钙黏蛋白片段对Cry1Ac蛋白的增效作用[J].植物保护,2016,42(3):45-51. |
| 小菜蛾钙黏蛋白片段对Cry1Ac蛋白的增效作用 |
| Synergistic effects of two cadherin gene fragments in functional region from Plutella xylostella to CrylAc protein toxicity |
| 投稿时间：2015-04-07 修订日期：2015-04-20 |
| DOI： |
| 中文关键词: 小菜蛾 钙黏蛋白片段 Cry1Ac 增效作用 表达 |
| 英文关键词:Plutella xylostella cadherin fragment CrylAc synergism expression |
| 基金项目:国家自然科学基金(31171861)；湖南省高等学校科学研究青年项目(12B065) |
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| 中文摘要: |
| 根据已报道的昆虫毒素结合区钙黏蛋白片段对Cry1Ac蛋白有增效作用, 本文以3龄小菜蛾幼虫为研究对象, 选取其钙黏蛋白相同功能区的两个片段, 将两个片段进行克隆。通过pGEX-6p-1载体, 在大肠杆菌中表达了功能区片段 PxCAD1及 PxCAD2。使用致死中浓度剂量的CrylAc蛋白(1 μg/mL)及较高浓度的 PxCAD1(556 μg/mL)与 PxCAD2(551.25 μg/mL)对小菜蛾幼虫进行体外复配生测, 结果表明, PxCAD1可使小菜蛾幼虫的致死率上升为85.56%, PxCAD2则不能增强CrylAc蛋白的杀虫活性; 而 PxCAD1与 PxCAD2本身对于小菜蛾幼虫并无毒性。研究结果为筛选有效的协同片段提供了理论依据, 对于揭示Bt杀虫蛋白的毒理机制和害虫对Bt杀虫蛋白的抗性机制具有重要意义。 |
| 英文摘要: |
| It was reported that peptide from toxin-binding region of cadherin receptor in some insects had a synergistic effect on Cry1Ac toxin. Two cadherin gene fragments (PxCAD1 and PxCAD2) from Plutella xylostella were cloned. The functional region fragments PxCAD1 and PxCAD2 were expressed in Escherichia coli by using vector pGEX-6p-1. Bioassays with CrylAc (1 μg/mL) in the presence of PxCAD1 (556 μg/mL) polypeptide and PxCAD2 (551.25 μg/mL) polypeptide were performed against P. xylostella larvae. The results showed that PxCAD1 caused a mortality of 85.56% to the larvae, while there was no significant enhancement of CrylAc toxicity to P. xylostella larvae with PxCAD2 peptide. Meanwhile, PxCAD1 and PxCAD2 alone were non-toxic to P. xylostella larvae. Our results provided theoretical basis for screening effective synergistic fragments, and had great significance in revealing the mechanism of Bt insecticidal protein and insect resistance to Bt. |
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