| 张水英1, 蒋春和2, 兰平秀1,3, 张雪峰4*, 李 凡1*.应用RT-PCR和dot-ELISA方法检测单头灰飞虱体内水稻条纹病毒[J].植物保护,2016,42(2):119-122. |
| 应用RT-PCR和dot-ELISA方法检测单头灰飞虱体内水稻条纹病毒 |
| Detection of Rice stripe virus in individual small brown planthoppers by RT-PCR and dot-ELISA |
| 投稿时间：2015-01-30 修订日期：2015-03-31 |
| DOI： |
| 中文关键词: 水稻条纹病毒 灰飞虱 RT-PCR dot-ELISA 检测 |
| 英文关键词:Rice stripe virus small brown planthopper RT-PCR dot-ELISA detection |
| 基金项目:云南省教育厅科学研究基金重大专项(ZD2012005); 云南省高校科技创新团队支持计划(云教科［2014］22号) |
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| 中文摘要: |
| 本文比较了RT-PCR和dot-ELISA两种方法对单头灰飞虱携带水稻条纹病毒(Rice stripe virus, RSV)的检出率、检测灵敏度和检测成本。结果表明, 两种方法均能检测到单头灰飞虱体内的RSV, RT-PCR检测单头灰飞虱体内RSV的阳性检出率比dot-ELISA方法低11.79%~15.77%, 但RT-PCR的检测灵敏度比dot-ELISA高10倍, RT-PCR技术的检测成本比dot-ELISA的高。结果暗示, 对于单头介体昆虫中的病毒检测, RT-PCR技术的检出率不一定比dot-ELISA的高。综合考虑后, 如对室外大批量灰飞虱进行带毒率检测时可采用dot-ELISA方法, 如需准确分析单头灰飞虱体内RSV时可采用RT-PCR方法。 |
| 英文摘要: |
| The detection rates, sensitivities and cost of RT-PCR and dot-ELISA methods were compared for the detection of Rice stripe virus (RSV) in individual small brown planthoppers (SBPH). The results indicated that RSV could be detected in single SBPH successfully by both methods. The RSV-positive detection rates in single SBPHs by RT-PCR were 11.79%15.77% lower than that by dot-ELISA; however, the sensitivity of RT-PCR was 10 times higher than that of dot-ELISA. The cost of RT-PCR was also higher. The results suggested that the detection rate of RSV in individual insect vectors by RT-PCR might be lower than that by dot-ELISA. Therefore, dot-ELISA is recommended for the detection of RSV in individual SBPHs when large-scale field samples need to be tested, while RT-PCR is used for accurate analysis of the virus in single SBPHs. |
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