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陈爱昌1, 魏周全1*,骆得功1,胡小平2.甘肃省定西市马铃薯黑痣病菌菌丝融合群的鉴定及药剂筛选[J].植物保护,2016,42(1):197-202.
甘肃省定西市马铃薯黑痣病菌菌丝融合群的鉴定及药剂筛选
Fungicide screening and identification of anastomosis groups of Rhizoctonia solani on potato
投稿时间:2014-12-09  修订日期:2015-02-06
DOI:
中文关键词:  马铃薯  黑痣病菌  融合群  鉴定  药剂筛选
英文关键词:potato  Rhizoctonia solani  anastomosis group  identification  fungicide screening
基金项目:甘肃省星火计划(1205NCXJ219)
作者单位
陈爱昌1, 魏周全1*,骆得功1,胡小平2 1. 甘肃省定西市植保植检站, 定西 743000
2. 西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室, 杨凌 712100 
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中文摘要:
      马铃薯黑痣病立枯丝核菌(Rhizoctonia solani)菌丝存在遗传分化现象。从定西市马铃薯产区采集茎部和薯块病样80份进行融合群测定。结果表明, 80株菌株中与标准菌株AG3融合的占总数的70.0%、与AG4-HG-Ⅱ融合的占15.0%、与AG2-1融合的占7.5%。为了有效控制马铃薯黑痣病, 采用生长速率法在室内选取9种杀菌剂进行药效评价。结果表明:30%噻呋酰胺悬浮剂EC50 最低, 为0.015 2 μg/mL; 其次是40%氟胺·异菌脲悬浮剂、3%多抗霉素可湿性粉剂和250 g/L嘧菌酯悬浮剂, 其EC50 分别为0.064 1、0.129 3和0.176 7μg/mL; EC50 最大的是25.75 %多抗·福美双可湿性粉剂, 为2.930 4 μg/mL。田间拌种, 30%噻呋酰胺悬浮剂和250 g/L嘧菌酯悬浮剂防效较好, 达70%以上, 值得在生产中推广。
英文摘要:
      Mycelium of potato black scurf (Rhizoctonia solani) existed genetic differentiation phenomenon. Anastomosis group (AG) identification of 80 diseased potato stems and tubers sampled from Dingxi City showed that 70% strains could fused with the standard strain AG3, 15% with AG4-HG-Ⅱ, and 7. 5% with AG2-1. Meanwhile, the toxicities of 9 fungicides to R. solani were evaluated by the mycelial growth rate method indoors. The results showed that 30% trifluzamide SC had the high toxicity with the EC50 value of 0.015 2 μg/mL, followed by 40% fluazinam·iprodione SC, 3% polyoxin WP and 250 g/mL azoxystrobin SC, with the values of 0.064 1, 0.129 3 and 0.176 7 μg/mL, respectively. 25.75% polyoxin·thiram WP was the lowest toxicity with the value of 2.930 4 μg/mL. Field trials demonstrated that 30% trifluzamide SC and 250 g/mL azoxystrobin SC by seed dressing had good control effect with the efficacy of more than 70%, which can be recommended in potato production.
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