| 邹娟*, 姚卓, 尚永华.柑橘炭疽病菌的生物学特性及枯草芽胞杆菌对其的抑制作用[J].植物保护,2016,42(1):61-67. |
| 柑橘炭疽病菌的生物学特性及枯草芽胞杆菌对其的抑制作用 |
| Biological characteristics of citrus pathogen Colletotrichum gloeosporioides and the inhibitory effect of Bacillus subtilis on the fungus |
| 投稿时间：2014-11-25 修订日期：2015-02-02 |
| DOI： |
| 中文关键词: 柑橘炭疽病 盘长孢状刺盘孢 枯草芽胞杆菌 抗菌活性 |
| 英文关键词:citrus anthracnose Colletotrichum gloeosporioides Bacillus subtilis antagonistic activity |
| 基金项目:湖南省重点学科建设项目资助(201142); 怀化学院科研项目(HHUY2014-03); 湖南省教育厅科研项目(11C0988) |
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| 中文摘要: |
| 近5年来, 湖南麻阳苗族自治县的柑橘种植园发生柑橘炭疽病。采集发病柑橘植株, 分离其病原菌, 从病原菌的培养性状和形态学特征、内转录区(ITS)序列分析和系统发育关系比较等方面对该病原菌进行了鉴定, 并探索了枯草芽胞杆菌(Bacillus subtilis)CGMCC 1.354对其的抑制作用。结果表明:该地区柑橘炭疽病菌为盘长孢状刺盘孢(Colletotrichum gloeosporioides)。枯草芽胞杆菌CGMCC 1.354对该病原菌具有较强的抑制作用, 其分泌的抑菌物质能抑制柑橘炭疽病菌菌丝生长和孢子萌发。 |
| 英文摘要: |
| Anthracnose on citrus has occurred in Mayang Miao Autonomous County, Hunan Province in the recent five years. The pathogen causing citrus anthracnose was isolated from citrus orchards of Mayang Miao Autonomous County. Based on cultural and morphological characteristics, ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence analysis and phylogenetic relationships with other species of Colletotrichum, the pathogen was identified as Colletotrichum gloeosporioides. Bacillus subtilis CGMCC 1.354 showed strong antagonistic activity against the pathogen, and the antifungal substance produced by B. subtilis CGMCC 1.354 strongly inhibited the hyphal growth and spore germination of C. gloeosporioides. |
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