| 王江1, 武奉慈2, 刘新颖1, 冯树丹1*, 宋新元2*.转基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟的抗性及对草甘膦的耐受性研究[J].植物保护,2016,42(1):45-50. |
| 转基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟的抗性及对草甘膦的耐受性研究 |
| Evaluation of transgenic maize ‘Shuangkang 12-5’ with complextraits of insect-resistance and glyphosate-resistance for theresistance to Ostrinia furnacalis and tolerance to glyphosate |
| 投稿时间：2015-08-13 修订日期：2015-10-15 |
| DOI： |
| 中文关键词: 转基因玉米 复合性状 亚洲玉米螟 抗虫性 除草剂耐受性 |
| 英文关键词:transgenic maize complex traits Ostrinia furnacalis insect resistance herbicide tolerance |
| 基金项目:国家自然科学基金(31301329); 转基因生物新品种培育科技重大专项(2016ZX08011-003); 黑龙江省自然科学基金(c201307) |
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| 中文摘要: |
| 通过田间接虫、室内生测以及田间植株喷施草甘膦试验, 在吉林省公主岭市综合评价了转cry1Ab/cry2Aj和G10evo-epsps基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟［Ostrinia furnacalis (Guenée)］的抗性以及对草甘膦的耐受性, 为‘双抗12-5’在吉林省产业化应用提供基础科学数据。结果表明, 与非转基因对照‘郑单958’相比, ‘双抗12-5’全生育期对亚洲玉米螟都具有良好的抗性, 达到高抗水平; 取食‘双抗12-5’心叶、雄穗及花丝等组织7 d后的亚洲玉米螟幼虫存活率为4%~12%, 而取食‘郑单958’各个组织的亚洲玉米螟幼虫存活率均高于96%。‘双抗12-5’在喷施推荐剂量(1 230 g/hm2)及在2倍推荐剂量(2 460 g/hm2)草甘膦情况下, 4周内均生长正常, 受害率为0; 对照‘郑单958’植株第一周即表现出明显的药害症状, 逐渐枯萎死亡, 受害率达到100%。综上所述, ‘双抗12-5’在吉林省表现出良好的抗虫性和除草剂耐受性。 |
| 英文摘要: |
| ‘Shuangkang 12-5’is a maize variety harbored the genes cry1Ab/cry2Aj and G10evo-epsps. Evaluation of the transgenic maize for the resistance to Asian corn borer［Ostrinia furnacalis (Guenée)］ and tolerance to glyphosate was assayed in Gongzhuling City, Jilin Province. Field and indoor bioassay demonstrated that the ‘Shuangkang 12-5’ exhibited a high resistance to Asian corn borer during the whole growth period, compared with non-transgenic variety ‘Zhengdan 958’. The survival rate of the larvae that fed on different tissues of ‘Shuangkang 12-5’ for 7 days were 4%-12%, while that fed on non-transgenic lines were above 96%. When treated with glyphosate at the recommended dose (1 230 g/hm2) and the double dose (2 460 g/hm2), all the transgenic lines survived in four weeks, but there was symptom of serious chemical damage in one week and the plant gradually died for the non-transgenic lines. Thus, the transgenic maize ‘Shuangkang 12-5’ presented good insect resistance and herbicide tolerance in Jilin Province. |
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