| 李巳夫1,2，唐涛2，刘雪源2，刘都才2，柏连阳3*，马国兰2，彭亚军2.利用贴牌水培法快速鉴定稗草对二氯喹啉酸的抗药性水平[J].植物保护,2015,41(6):144-149. |
| 利用贴牌水培法快速鉴定稗草对二氯喹啉酸的抗药性水平 |
| Rapid identification of resistance level of barnyardgrass (Echinochloa crusgalli) to quinclorac using water culture method with label cards (WCMLC) |
| 投稿时间：2014-10-24 修订日期：2015-01-29 |
| DOI： |
| 中文关键词: 稗草 贴牌水培法 二氯喹啉酸 甄别剂量 抗性倍数 |
| 英文关键词:Echinochloa crusgalli water culture method with label cards (WCMLC) quinclorac screening dose resistance factor |
| 基金项目:国家公益性行业(农业)科研专项(201303031)；国家自然科学基金项目(31201530) |
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| 中文摘要: |
| 本文通过室内贴牌水培法及整株法，快速鉴定并验证了2013年采自湖南省不同地区稻田的50个稗草生物型对二氯喹啉酸的抗药性，试验结果表明：贴牌水培法所用的二氯喹啉酸甄别剂量为80 mg/L；稗草死亡率越低，其抗性倍数(resistance factor，RF)越高，其中死亡率为0时，整株法测定的RF值高达896.07~1209.38；贴牌水培法测定的稗草样本对二氯喹啉酸的ED50远低于整株法，但不同稗草生物型对二氯喹啉酸的抗性水平趋势高度吻合。笔者认为以贴牌水培法测定的死亡率小于40%的稗草样本对二氯喹啉酸的抗性风险较高，值得深入研究。 |
| 英文摘要: |
| In the present study, 50 biotypes of barnyardgrass, Echinochloa crusgalli (L.) Beauv., were collected from different rice fields of Hunan Province in 2013, and their resistance levels to quinclorac were conducted using water culture method with label cards (WCMLC) and whole plant method (WPM). The bioassay results showed that the screening dose of quinclorac was 80 mg/L via WCMLC.The lower mortalities of the tested E. crusgalli, the higher values of resistance factor (RF). RF values were 896.07-1 209.38 as for 0 of mortality. Comparing ED50 value between WCMLC and WPM, similar resistance level trends of E. crusgalli to quinclorac were observed. The ED50 value determined by WCMLC was remarkably lower than that of WPM.There was a higher resistant risk to 80 mg/L of quinclorac when the mortalities of E. crusgalli biotypes was lower than 40% using WCMLC. Therefore, further study was worthy for carrying out in these biotypes. |
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