| 朱航1，王雅菲1，周小毛1,2﹡.小菜蛾ABCG2 mRNA表达特征及茚虫威对其表达量的影响[J].植物保护,2015,41(6):117-121. |
| 小菜蛾ABCG2 mRNA表达特征及茚虫威对其表达量的影响 |
| Characteristics of mRNA relative expression of ABCG2 in Plutella xylostella and the effect of indoxacarb on its expression |
| 投稿时间：2014-11-05 修订日期：2015-02-10 |
| DOI： |
| 中文关键词: 小菜蛾 茚虫威 ABCG2 mRNA 荧光定量PCR |
| 英文关键词:Plutella xylostella indoxacarb ABCG2 mRNA qRT-PCR |
| 基金项目:国家自然科学基金项目(31371966) |
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| 中文摘要: |
| 采用实时荧光定量RT-PCR技术，测定了小菜蛾不同龄期、4龄幼虫不同组织ABCG2 mRNA相对表达量的差异，并进一步检测了茚虫威的LC50剂量对小菜蛾3龄幼虫ABCG2 mRNA表达量的影响。结果表明，不同发育阶段小菜蛾ABCG2 mRNA相对表达量在雄性成虫中最高，明显高于其他几个发育阶段，分别是3龄的5.63倍、4龄的3.67倍、蛹期的7.36倍、雌虫的3.51倍。4龄幼虫的ABCG2 mRNA在中肠中的表达量最高，分别是马氏管、精巢、残余体的3.12倍、8.96倍、7.33倍，在头和表皮的表达量最低；使用茚虫威的LC50浓度处理小菜蛾3龄幼虫后，ABCG2 mRNA的表达量逐步上升。研究结果为进一步探究茚虫威在小菜蛾体内的代谢与ABCG2之间的关系提供一定的参考。 |
| 英文摘要: |
| qRT-PCR was used to determine the expression of ABCG2 mRNA at different developmental stages of Plutella xylostella (L.) and in different tissues of its 4th-instar larvae. And influence of LC50 of indoxacarb on expression of ABCG2 mRNA in the 3rd-instar larvae was also investigated. The results showed that the expression of ABCG2 mRNA in male adults of P. xylostella was significantly higher than those in other stages, which was 5.63, 3.67, 7.36 and 3.51 times of that in the 3rd-, 4th-instar larvae, the pupal stage, and the female adults, respectively. In different tissues of the 4th-instar larvae, the mid-gut had the highest expression, which was 3.12, 8.96 and 7.33 times of that in the Malpighian tubule, testis and carcass, respectively. The expression of ABCG2 mRNA in the head and integument were the lowest. To further identify the effect of indoxacarb on ABCG2 mRNA, the relative expression of ABCG2 mRNA in 3rd-instar larvae treated by indoxacarb was also analyzed. The results suggested that the expression of ABCG2 increased gradually over time. The results are helpful for further study of the relationship of ABCG2 expression and indoxacarb resistance in P. xylostella. |
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