| 刘昌燕1，肖炎农2，吴小微2，李莉1，陈宏伟1，刘良军1，万正煌1*.绿豆叶斑病病原鉴定及生物学特性研究[J].植物保护,2015,41(6):83-87. |
| 绿豆叶斑病病原鉴定及生物学特性研究 |
| Pathogen identification and biological characteristics of mungbean Cercospora leaf spot |
| 投稿时间：2014-09-22 修订日期：2014-12-04 |
| DOI： |
| 中文关键词: 绿豆 叶斑病 变灰尾孢 鉴定 生物学特性 |
| 英文关键词:mung bean leaf spot disease Cercospora canescens identification biological characters |
| 基金项目:国家现代农业产业技术体系(CARS-09); 科技部国际合作重点项目(2011DFB31620); 湖北省农业科学院青年科学基金项目(2014NKYJJ35) |
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| 中文摘要: |
| 对绿豆尾孢菌叶斑病菌进行形态学和分子特征鉴定，结果表明，分离物在V8培养基上菌落近圆形，菌落边缘泛紫色，分生孢子无色；用通用引物18SF和28SR扩增6个分离物的rDNA-ITS部分序列和测序，并到GenBank (登录号：KM435076) 数据库进行比对，6个分离物与变灰尾孢分离物序列相似性为99%，基于形态和分子特征将绿豆叶斑病病原菌鉴定为变灰尾孢。致病性测定结果表明，6个分离物对绿豆‘中绿一号’叶片均致病且存在致病力差异。不同培养基、温度和pH对病原菌生长的影响研究表明，V8培养基最适宜该病菌生长，病菌生长的最适温度为25℃，最适pH为6。 |
| 英文摘要: |
| The morphology and molecular characterization of mung bean Cercospora leaf spot were studied. The results indicated that the colony of isolates was circular in V8 medium, the edge was purple and the conidia were colorless. The partial rDNA-ITS sequences of six isolates were amplified with universal primers 18SF and 28SR.The obtained ITS sequences (GenBank accession No. KM435076) were aligned with GenBank and showed 99% similarity with isolates of Cercospora canescens. Based on morphological and molecular characteristics, the pathogen of mung bean leaf spot was identified as C. canescens. Pathogenicity test results showed that all mung bean cv ‘Zhonglv No.1’ leaves showed dark brown lesions when inoculated with six isolates and had virulence differences. The influences of different media, temperatures and pH values on the growth of pathogen were observed. V8 was the optimum culture medium for pathogen growth, at 25℃ and pH 6. |
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