| 黄娜，周莉质，费丹，施扬玮，徐辉，檀根甲*.枯草芽胞杆菌胞外抗菌蛋白的初步分离及性质的研究[J].植物保护,2015,41(6):49-54. |
| 枯草芽胞杆菌胞外抗菌蛋白的初步分离及性质的研究 |
| Primary separation of an extracellular antifungal protein from Bacillus subtilis |
| 投稿时间：2014-11-09 修订日期：2015-03-04 |
| DOI： |
| 中文关键词: 枯草芽胞杆菌 凝胶电泳 理化性质 质谱鉴定 |
| 英文关键词:Bacillus subtilis PAGE-polyacrylamide gel electrophoresis physiochemical property mass spectrum identification |
| 基金项目:安徽省教育厅自然科学重点科研项目(KJ2007A095) |
|
| 摘要点击次数: 906 |
| 全文下载次数: 1242 |
| 中文摘要: |
| 土壤中分离的枯草芽胞杆菌，其发酵液对苹果炭疽病菌具有很强的抑制作用。为了确定其抑菌物质的主要成分，本试验采用硫酸铵分级沉淀获得无菌滤液中的抗菌蛋白粗提物，超滤后经过聚丙烯酰胺凝胶电泳，胶带回收进行分离鉴定，得到1种具有抑菌活性的蛋白条带。该蛋白对供试的6种植物病原真菌具有抑制作用，该蛋白的活性表现在80℃，30 min仍有抑菌作用，对pH(4~7)具有较广的适应范围 、另外对紫外照射(0~12 h)、抑制剂、有机溶剂均不敏感。质谱鉴定该条带含有两种蛋白，分别为假定蛋白BSU35980 gi|16080651,相对分子量11 079 Da,等电点4.78,匹配率为68%;丝氨酸羟甲基转移酶gi|16080743,相对分子量为45 575 Da,等电点为5.56,匹配率为67%。显微观察发现抑菌蛋白可以使菌丝发生断裂、扭曲、畸形、肿大。 |
| 英文摘要: |
| Bacillus subtilis was isolated from the soil, and the fermentation liquid had strong inhibitory action against Colletotrichum gloeosporioides. In order to identify the antimicrobial compounds, the antimicrobial substances were isolated and purified by (NH4)2SO4 fractional precipitation, ultrafiltration, PAGE polyacrylamide gel electrophoresis, and tape reciting. The results showed that the purified active protein had inhibitory action to 6 plant pathogenic fungi. The protein was found to be stable at 80℃ for 30 min. The inhibitory activity of the protein was observed in the range of pH value from 4 to 7, and not sensitive to ultraviolet radiation, inhibitor and organic solvents. Two kinds of proteins contained in this stripe were identified by mass spectrum. They were the hypothetical protein BSU35980 gi|16080651, with a relative molecular mass of 11 079 Da and isoelectric point (pI) of 4.78, and a matching ratio of 68%, and serine hydroxymethyltransferase gi|16080743, with a relative molecular mass of 45 575 Da and isoelectric point (pI) of 5.56, and a matching ratio of 67%. Microscope observation revealed that the protein could break up and enlarge the mycelia. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
