| 杜广祖1, 杨卫秋1, 周跃能1, 2, 和淑琪1, 陈 斌1*, 桂富荣1, 李正跃1.黄绿绿僵菌KM1104对灰飞虱的毒力[J].植物保护,2015,41(5):207-211. |
| 黄绿绿僵菌KM1104对灰飞虱的毒力 |
| Virulence of Metarhizium flavoviride strain KM1104 against Laodelphax striatellus (Fallén) |
| 投稿时间：2014-06-24 修订日期：2014-09-12 |
| DOI： |
| 中文关键词: 灰飞虱 黄绿绿僵菌 致死中时 致死中浓度 |
| 英文关键词:Laodelphax striatellus Metarhizium flavoviride LT50 LC50 |
| 基金项目:国家重点基础研究发展计划("973")项目(2011CB100404); 国家科技支撑计划项目(2012BAD40B01); 国家自然科学基金 (30860005); 公益性行业(农业)科研专项(20100301); 2014年云南省博士研究生学术新人奖资助项目 |
| 作者 | 单位 | | 杜广祖1, 杨卫秋1, 周跃能1, 2, 和淑琪1, 陈 斌1*, 桂富荣1, 李正跃1 | 1.云南农业大学植物保护学院, 云南省微生物发酵工程研究中心有限公司, 农业生物多样性应用技术研究国家工程中心, 昆明 650201
2. 云南省河口县植检植保站, 河口 661300 |
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| 中文摘要: |
| 为了筛选防治灰飞虱的高毒力生防真菌菌株及明确黄绿绿僵菌对灰飞虱的侵染致病温度条件, 室内测定了黄绿绿僵菌KM1104菌株在24~30 ℃下对灰飞虱成虫及3龄若虫的毒力。结果表明:黄绿绿僵菌菌株KM1104对灰飞虱成虫和3龄若虫均具有较强的毒力, 其中对成虫的毒力高于3龄若虫, 在24~30 ℃下对成虫和3龄若虫的LC50分别为1.45×103~7.14×104和7.25×103~3.69×105孢子/mL，致死中时LT50分别为3.02~11.53 d和6.85~11.58 d。不同温度下黄绿绿僵菌KM1104菌株对灰飞虱的毒力不同, 其中30 ℃下毒力最强, 对成虫和3龄若虫的致死中浓度LC50分别为1.45×103和7.25×103孢子/mL, 接种1.01×105~1.01×108孢子/mL后对成虫和3龄若虫的致死中时LT50分别为3.02~6.86 d和6.85~9.35 d。综合以上结果, 黄绿绿僵菌菌株KM1104具有良好的灰飞虱生防制剂开发潜力, 且在30 ℃下使用致病力最强。 |
| 英文摘要: |
| In order to screen the higher virulent entomopathogenic fungi and the optimal temperature for its infection, the virulence of Metarhizium flavoviride strain KM1104 against the adults and 3rd instar nymph of Laodelphax striatellus was tested at different temperatures. The results showed that the strain KM1104 was virulent against the adult and 3rd instar nymph of L.striatellus. The virulence against the adult was significantly higher than that against the 3rd instar nymph. The LC50 value for the adult and 3rd instar nymph was 1.45×103~7.14×104 and 7.25×103~3.69×105 spores/mL, and the LT50 value was 3.02~11.53 d and 6.85~11.58 d, respectively. The virulence was significantly different at different temperature, the highest virulence was at temperature of 30 ℃, the LC50 value for the adult and 3rd instar nymph was 1.45×103and 7.25×103 spores/mL, the LT50 value for the adult and 3rd instar nymph after inoculation of 1.01×105~1.01×108 spores/mL was 3.02~6.86 d and 6.85~9.35 d, respectively. Considering all the above, M. flavoviride strain KM1104 had great potential for L. striatellus control, and the highest virulence was at 30 ℃. |
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