| 霍世英1，徐玉梅1，高俊明1* ，王建明1，赵增旗2.山西短颈剑线虫(Xiphinema brevicollum)形态与rDNA 分子特征[J].植物保护,2015,41(5):134-139. |
| 山西短颈剑线虫(Xiphinema brevicollum)形态与rDNA 分子特征 |
| Morphological and molecular characterization of Xiphinema brevicollum from Shanxi |
| 投稿时间：2014-06-29 修订日期：2014-10-10 |
| DOI： |
| 中文关键词: 剑线虫 形态 ITS1 ITS2 28S RNA |
| 英文关键词:Xiphinema morphology ITS1 ITS2 28S RNA |
| 基金项目:山西省重点学科建设专项资金; 山西省“百人计划”项目 |
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| 中文摘要: |
| 采用浅盘分离法在山西太谷松树根际土壤中分离得到一种剑线虫, 通过Seinhorst脱水制片后利用显微镜观察其形态特征并进行测量, 其主要特征为:雌性虫体经热杀死后呈腹面弯曲的“C”形, 体长1.71~2.26 mm, 两端渐细; 齿针全长127.3~140.8 μm; a(体长/体宽)为35.9~51.4 μm, b(体长/食道外宽)为5.8~8.7 μm, c(体长/尾长)为50.7~103 μm。前卵巢与后卵巢长短相近, 尾形近似圆锥形。未见雄虫。对该线虫的rDNA ITS1, rDNA ITS2和28S RNA序列进行扩增、测序及系统进化分析, 结合形态学特征进行鉴定, 将分离到的剑线虫鉴定为短颈剑线虫(Xiphinema brevicollum), 这是山西首次发现报道剑线虫属的一个种。 |
| 英文摘要: |
| In the present study, soil samples were collected from rhizosphere soil of pine trees in Taigu County of Shanxi Province.Nematodes were extracted from soil samples by a modified Baermann funnel method. Specimens used for morphological and morphometric studies were prepared as permanent slides using the Seinhorst’s method. Microscopic observation demonstrated that the female body was usually strongly curved, C shaped, with the body length of 1.71-2.26 mm and the entire odontostyle length of 127.3-140.8 μm. a(body length/body diameter at mid body), b(body length/pharyngeal bulb diam.) and c(body length/ tail length)were 35.9-51.4 μm, 5.8-8.7 μm, 50.7-103 μm, respectively. The length of anterior uterus was similar to the posterior one. Tail regularly was presented to be short conical. Male was not observed. Based on the morphological characteristic and sequences of rDNA ITS1, rDNA ITS2 and 28S RNA, the nematode was identified to be Xiphinema brevicollum (Nematoda: Longidoridae), which was firstly reported in Shanxi Province. |
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