| 高树广1,2，赵辉1，李伟峰2，王瑞霞2，倪云霞1，杨修身1，刘红彦1*，杨光宇2.芝麻茎点枯病菌两种细胞壁降解酶活性分析[J].植物保护,2015,41(5):75-78. |
| 芝麻茎点枯病菌两种细胞壁降解酶活性分析 |
| Analysis of the enzyme activity of two kinds of cell wall-degrading enzyme of Macrophomina phaseolina in sesame |
| 投稿时间：2014-08-20 修订日期：2014-11-04 |
| DOI： |
| 中文关键词: 芝麻 茎点枯病菌 漆酶 聚甲基半乳糖醛酸酶 |
| 英文关键词:sesame Macrophomina phoseolina Lac PMG |
| 基金项目:现代农业产业技术体系建设专项资金(CARS-15-1-05) |
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| 中文摘要: |
| 为明确芝麻茎点枯病菌漆酶(Lac)和聚甲基半乳糖醛酸酶(PMG)活性, 采用分光光度法测定了9株芝麻茎点枯病菌漆酶活性和聚甲基半乳糖醛酸酶活性。结果表明, 芝麻茎点枯病菌胞内、胞外均能检测到漆酶, 不同菌株之间漆酶活性差异显著; 芝麻茎点枯病菌体外培养能持续产生聚甲基半乳糖醛酸酶, 并且不同菌株之间聚甲基半乳糖醛酸酶活性差异显著。 |
| 英文摘要: |
| The purpose of this study is to examine and measure the enzyme activity. The Lac activity and PMG activity of nine strains of Macrophomina phaseolina were tested by spectrophotom.The results demonstrated that intracellular Lac activity and extracellular Lac activity could be detected in M.phaseolina, and the Lac activity difference was significant among strains. PMG could be produced by M.phaseolina continuously during ten days, and the PMG activity difference was also significant among strains.This work is helpful for the study of pathogenic mechanism. |
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