| 徐广增, 李伟, 王新红, 刘守柱*, 王桂清.斜纹夜蛾酚氧化酶酶学特性研究[J].植物保护,2015,41(3):45-49. |
| 斜纹夜蛾酚氧化酶酶学特性研究 |
| Characteristics of phenoloxidase from Spodoptera litura |
| 投稿时间：2014-04-18 修订日期：2014-05-29 |
| DOI： |
| 中文关键词: 斜纹夜蛾 酚氧化酶 酶学特性 稳定性 酶活性 |
| 英文关键词:Spodoptera litura phenoloxidase enzyme characteristics stability enzyme activity |
| 基金项目:聊城大学博士科研启动基金（31805）; 聊城大学“园艺植物病虫害控制技术研究”重点建设项目 |
|
| 摘要点击次数: 831 |
| 全文下载次数: 806 |
| 中文摘要: |
| 以邻苯二酚为底物, 研究了斜纹夜蛾幼虫酚氧化酶的酶学特性。结果表明：斜纹夜蛾幼虫酚氧化酶的最适pH在6.7~7.0之间, pH 6.8时, 稳定性最好; 最适温度为30 ℃, 20~35 ℃之间, 稳定性较好; 在3 mL的测活体系中, 当酶量为0.1 mL时, 酶促反应速度随底物浓度升高而增大, 当底物浓度为10 mmol/L时, 酶促反应最快, 超过10 mmol/L时, 酶促反应趋于平衡; 以邻苯二酚为底物时, 斜纹夜蛾酚氧化酶的Km值为7.980 mmol/L, Vm为204.082 ΔA/mg?10 s。 |
| 英文摘要: |
| The properties of phenoloxidase (PO) from Spodoptera litura (Fabricius) were determined with catechol as substrate. The results showed that the optimum pH for PO from S. litura was 6.7-7.0, and the enzyme had the maximum stability at pH 6.8. PO showed the highest activity at 30 ℃, while it had the best thermal stability at 20-35 ℃. When the amount of enzyme was limited to 0.1 mL in a total of 3 mL assay mixture, the reaction velocity increased with the concentration of substrate, and when the final concentration of catechol was 10 mmol/L, the velocity reached the highest, and then kept constant; the Km and Vm values were 7.980 mmol/L and 204.082 ΔA/mg?10 s, respectively, with catechol as substrate. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
