| 张晶晶1, 2, 黄亚丽1*, 马宏1, 贾振华1, 史延茂1, 宋水山1*.木霉Tr-92菌株厚垣孢子发酵条件的优化[J].植物保护,2015,41(3):25-29. |
| 木霉Tr-92菌株厚垣孢子发酵条件的优化 |
| Optimization of liquid fermentation conditions for production of Trichoderma Tr-92 chlamydospores |
| 投稿时间：2014-04-18 修订日期：2014-08-14 |
| DOI： |
| 中文关键词: 木霉 厚垣孢子 液体发酵 培养基 发酵条件 |
| 英文关键词:Trichoderma chlamydospores fermentation in liquid culture medium fermentation condition |
| 基金项目:河北省自然科学基金（C2010001565）; 河北省省级科技计划专项工作类项目（13256504D） |
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| 中文摘要: |
| 利用单因素试验、正交试验方法对木霉Tr-92厚垣孢子发酵培养基及培养条件进行优化, 筛选获得了适合此菌株厚垣孢子产生的最佳培养基组成为：草炭2.5%, 玉米浆4.5%, 葡萄糖2%, 酵母膏0.5%; 最佳培养条件为：接种量3%, 装液量75 mL/250 mL, 转速为180 r/min, 初始pH 5.0, 温度28 ℃, 培养时间7 d。在此培养条件下, 木霉Tr-92菌株厚垣孢子产量达到3.01×108个/mL。与优化前相比, 厚垣孢子产量增长130.80％。通过培养条件优化确定了适合木霉Tr-92菌株产生厚垣孢子的条件, 为高效木霉厚垣孢子生防菌剂的研制奠定了基础。 |
| 英文摘要: |
| The optimal liquid fermentation conditions for production of Trichoderma Tr-92 chlamydospores were investigated by single factor test and orthogonal test. The results indicated that the optimal conditions for production of Trichoderma Tr-92 chlamydospores were as followed: 2.5% peat, 4.5% maize steep liquor, 2% glucose, 0.5% yeast extract, 3% inoculum, 180 r/min, 28 ℃, initial pH 5. 0, and 75 mL liquid medium per 250 mL flask. The actual yield of chlamydospores was 3.01×108spores/mL after 7 days. The chlamydospore production increased by 130.80% compared with the control. |
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