| 徐希宝, 张靖, 芮昌辉*.沉默GSTs基因影响棉铃虫对甲氧虫酰肼的敏感性[J].植物保护,2015,41(2):49-54. |
| 沉默GSTs基因影响棉铃虫对甲氧虫酰肼的敏感性 |
| Effects of silencing GSTs genes on the susceptibility of Helicoverpa armigera to methoxyfenozide |
| 投稿时间：2014-03-06 修订日期：2014-05-19 |
| DOI： |
| 中文关键词: 甲氧虫酰肼 棉铃虫 谷胱甘肽S-转移酶 RNA干扰 实时定量PCR |
| 英文关键词:methoxyfenozide Helicoverpa armigera glutathione S-transferase RNAi qRT-PCR |
| 基金项目:国家“863”计划主题项目(2012AA1015027); 公益性行业(农业)科研专项(201203038) |
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| 中文摘要: |
| 利用RNAi技术对GSTs基因进行沉默处理, 明确了沉默棉铃虫GSTs基因可以影响其对甲氧虫酰肼的敏感性。结果表明, 通过饲喂siRNA在1 d内就可以有效干扰GSTs基因表达, 进而降低了GSTs酶活性; 停止饲喂siRNA后, RNA干扰效果并不会立刻消失; 沉默GSTs基因后, 棉铃虫对甲氧虫酰肼的敏感性显著提高。 |
| 英文摘要: |
| GSTs genes in Helicoverpa armigera were silenced by RNAi technique and the effects of silencing GSTs genes on the susceptibility of H.armigera to methoxyfenozide were investigated. The results showed that the expression level of GSTs genes markedly reduced from the first day by feeding siRNA, and then the specific activities of GSTs reduced. The effect of silencing would not disappear immediately after stopping feeding siRNA. The susceptibility of H. armigera to methoxyfenozide increased after GSTs genes being silenced. |
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