| 杨震发1, 袁呈山2, 杨顺义1*, 柳利龙1, 杨晋伟2, 沈慧敏1.黄花棘豆抑制植物病原菌的活性成分研究[J].植物保护,2014,40(6):59-64. |
| 黄花棘豆抑制植物病原菌的活性成分研究 |
| Antifungal activity and isolation of active ingredients in Oxytropis ochrocephala |
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| DOI： |
| 中文关键词: 黄花棘豆 活性跟踪 分离鉴定 抑菌成分 紫檀烷 |
| 英文关键词:Oxytropis ochrocephala bioassay-guided method isolation and identification active ingredient pterocarpan |
| 基金项目:甘肃省农业生物技术研究与应用开发项目（GNSW-2004-08）; 甘肃省教育厅基金项目(032-06) |
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| 中文摘要: |
| 为了测定黄花棘豆(Oxytropis ochrocephala Bunge)提取物对常见病原真菌的活性, 明确其中的有效成分, 以黄花棘豆为材料, 结合生物活性追踪法, 对黄花棘豆的抑菌活性物质进行分离, 并利用现代波谱学技术进行结构鉴定。结果表明, 黄花棘豆乙醇提取物对供试4种病原真菌均表现出较强的活性, 10 mg/mL对番茄灰霉病菌的抑菌率达到82.08 %; 粗提物萃取后的二氯甲烷相和乙酸乙酯相为其活性部分, 10 mg/mL对番茄灰霉病菌的抑菌率分别为84.30 %和83.13 %; 从合并后的二氯甲烷和乙酸乙酯相中分离、鉴定得到了6个化合物, 其中3-羟基-4, 9-二甲氧基紫檀烷、3-羟基-9-甲氧基紫檀烷和3-羟基-8, 9-二甲氧基紫檀烷对供试病原菌表现较好的抑制活性; 3-羟基-4, 9-二甲氧基紫檀烷对番茄灰霉病菌的EC50值为10.42 μg/mL, 3-羟基-8, 9-二甲氧基紫檀烷对黄瓜枯萎病菌的EC50值为27.09 μg/mL。黄花棘豆提取物对病原真菌具有较好的抑制活性, 紫檀烷类化合物为其主要的活性成分, 具备进一步开发和应用的潜力。 |
| 英文摘要: |
| In order to find new botanical antifungal compounds, antifungal ingredients of Oxytropis ochrocephala Bunge were isolated. Using bioassay-guided fractionation methods, antifungal ingredients were isolated and monomeric compounds were identified by modern spectroscopy techniques from O. ochrocephala. All data were processed statistically by using SPSS 17.0. Ethanol extract of O. ochrocephala showed strong activity against the selected pathogenic fungi with an inhibition ratio of 82.08 % at the concentration of 10 mg/mL. The methylene chloride and ethyl acetate phases displayed a significant antifungal activity against Botrytis cinerea, with an inhibition ratio of 84.30% and 83.13%, respectively. Six compounds were isolated and identified through comparing the data obtained via spectroscopic methods and the values reported in the literature. The screening of antifungal activity showed that 3-hydroxy-4, 9-dimethoxypterocarpan, 3-hydroxy-9-dimethoxy pterocarpan and 3-hydroxy-8, 9-dimethoxypterocarpan had higher EC50 on tested fungi. The EC50 of 3-hydroxy-4, 9-dimethoxypterocarpan against B. cinerea was 10.42μg/mL and the EC50 of 3-hydroxy-8, 9-dimethoxypterocarpan against Fusarium oxysporum was 27.09μg/mL. O. ochrocephala could be used as a biological antifungal agent and pterocarpan compounds are its significant antifungal ingredients. |
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