| 陆然, 王媛媛, 朱晓峰, 段玉玺, 陈立杰*.八角根围土壤真菌种类鉴定及其对南方根结线虫的活性评价[J].植物保护,2014,40(6):42-46. |
| 八角根围土壤真菌种类鉴定及其对南方根结线虫的活性评价 |
| Identification of the fungi from the rhizosphere of anise and evaluation of their activities on Meloidogyne incognita |
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| DOI： |
| 中文关键词: 八角 土壤真菌 南方根结线虫 活性筛选 种类鉴定 |
| 英文关键词:Illicium verum soil fungi Meloidogyne incognita activity screening identification of fungus |
| 基金项目:公益性行业(农业)科研专项(201103018); 国家自然科学基金(31171823); 沈阳农业大学天柱山学者资助计划 |
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| 中文摘要: |
| 采用平板稀释法分离广西八角根围土壤真菌13个属1 329株, 利用贝氏皿浸渍法对其发酵液进行了杀线虫活性筛选, 计算其对南方根结线虫卵孵化抑制率及对2龄幼虫(J2)致死率的影响。菌株Gisf326、Gisf495和Gisf415发酵液原液对南方根结线虫卵孵化的相对抑制率分别为96.67%、83.33%和73.33%, 发酵液20倍稀释液对J2校正死亡率分别为93.13%、87.81%和93.38%; 经形态学鉴定和ITS序列分析, 3种真菌分别为淡紫拟青霉( Paecilomyces lilacinus )、黑曲霉( Aspergillus niger ) 和日本曲霉( A. japonicus )。本研究为植物线虫病害的防治提供了新的生防真菌资源。 |
| 英文摘要: |
| Soil fungi were isolated by dilution method from the rhizosphere of anise（Illicium verum）in the Guangxi Zhuang Autonomous Region. A total of 1 329 strains were obtained, belonging to 13 genus. After culturing and fermenting some of the dominant fungi strains, we screened their nematicidal activities with fermentation metabolite immersed in the Bayesian dish. Through the evaluation of hatching rate of eggs and the corrected mortality of the second stage juveniles (J2), we got 3 high-activity fungal strains, namely Gisf326, Gisf495 and Gisf415, which were identified as Paecilomyces lilacinus, Aspergillus niger and A. japonicus based on morphology and ITS sequences. The relative inhibition rates of Gisf326, Gisf495 and Gisf415 in fermentation metabolite against M. incognita eggs were 96.67%, 83.33% and 73.33%. In 20-fold dilution metabolite, the corrected mortality rates of J2 were 93.13%, 87.81% and 93.38%. The research provides new biocontrol resources for plant nematodes. |
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