| 沈登荣1,2, 宋文菲3, 袁盛勇1, 田学军1, 和绍禹4, 张宏瑞2*.基于mtDNA-COⅠ的云南西花蓟马的遗传分析[J].植物保护,2014,40(5):75-79. |
| 基于mtDNA-COⅠ的云南西花蓟马的遗传分析 |
| Genetic analysis of Frankliniella occidentalis based on mtDNA-COⅠ in Yunnan, China |
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| DOI： |
| 中文关键词: 西花蓟马 mtDNA-COⅠ 遗传分化 复合种群 |
| 英文关键词:Frankliniella occidentalis mtDNA-COⅠ genetic differentiation composite population |
| 基金项目:公益性行业(农业)科研专项(201103026, 200803025); 云南省教育厅科学研究基金重点项目(2013Z148); 红河学院植物保护硕士点建设项目; 云南省高校农作物优质高效栽培与安全控制重点实验室项目 |
| 作者 | 单位 | | 沈登荣1,2, 宋文菲3, 袁盛勇1, 田学军1, 和绍禹4, 张宏瑞2* | 1. 红河学院生命科学与技术学院, 蒙自 661100
2. 云南农业大学植物保护学院, 昆明 650201
3. 云南省农业科学院蚕桑蜜蜂研究所, 蒙自 661101
4. 云南农业大学东方蜜蜂研究所, 昆明 650201 |
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| 中文摘要: |
| 西花蓟马是我国重要的入侵害虫之一, 为了解其不同地理种群的遗传分化, 测定了云南6个地理种群141个个体的mtDNA-COⅠ基因片段。结果表明：在比对的433 bp基因片段中, 变异位点19个, 共检测出5个共享单倍型, 单倍型间出现了明显的遗传分化, 证实了云南西花蓟马为一个复合种群。地理种群间的遗传距离较低, 遗传距离与地理距离无显著相关性。各地理种群西花蓟马的遗传分化指数 Fst 为-0.021 19, 种群间变异多为负值, 表明云南西花蓟马种群遗传变异主要来自于种群内部, 种群间未发生明显的遗传分化。 |
| 英文摘要: |
| Frankliniella occidentalis (Pergande) is an important invasive pest in China. In order to understand the genetic differentiation among six geographical populations of F. occidentalis, mtDNA-COⅠsequences of 141 individuals were determined. The results showed that there were 19 variable sites in the 433 bp gene fragment. Five shared haplotypes were detected, and these haplotypes had an obvious genetic differentiation, which confirmed that F. occidentalis was a composite population in Yunnan. The genetic distance was low among populations, and there was no significant correlation between genetic distance and geographic distance. The Fst value was -0.021 19 among geographical populations, indicating that genetic variation was mainly derived from within the population, and the genetic differentiation among populations was not significant. |
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