| 李春杰1, 谭国忠1, 王义2, 许艳丽1*.寒区昆虫病原线虫冷冻储存条件优化[J].植物保护,2014,40(5):49-54. |
| 寒区昆虫病原线虫冷冻储存条件优化 |
| Optimizing freezing storage conditions for the entomopathogenic nematode isolated from the cold region in China |
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| DOI： |
| 中文关键词: 昆虫病原线虫 冷冻储存 存活率 侵入力 |
| 英文关键词:entomopathogenic nematode freezing storage survival rate infective ability |
| 基金项目:国家科技计划农业成果转化项目(2007GB24910482); 中国科学院知识创新工程重要方向项目(KSCX2-YW-N-092); 黑龙江省青年科学基金项目(QC2010070); 哈尔滨市科技创新人才项目(2009RFQYN092) |
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| 中文摘要: |
| 以我国高寒地区分离、筛选出的高致病力昆虫病原线虫嗜菌异小杆线虫(Heterorhabditis bacteriophora HBN)为研究对象, 对其冷冻储存条件进行优化。结果显示, 介质中不同含水量对线虫HBN的冷冻存活率有着显著的影响; 其中含水量为50%的蛭石中线虫回收率最高, 为87.4%; 冷冻储存后的线虫经4 ℃ 24 h+室温1 h解冻利于线虫冷冻休眠后复苏; 线虫经阶段性降温较直接降温至-4 ℃的冷冻存活率提高31.6百分点; 储存介质蛭石中采用灭菌自来水较适于HBN线虫在-4 ℃冷冻存活; 7 d内冷冻储存增强了HBN线虫对寄主昆虫的侵入能力。 |
| 英文摘要: |
| One high virulence strain of EPNs, Heterorhabditis bacteriophora HBN (HBN) with cold tolerance isolated from Harbin City was used in the laboratory in this study. Storage substance species and moisture, cooling rate, thawing ways and osmotic pressure of solution in the substances were optimized. The results showed that freezing survival of HBN were significantly affected by the substances with different water contents. Recycling coefficient of H. bacteriophora HBN in the vermiculite with water content of 50% was the highest (87.4%). The way of freezing HBN at room temperature for 1h following 4 ℃ for 24h was in favor of thawing of freezing dormancy. Freezing survival rate of HBN by phasic cooling pattern was increased by 31.6 percent than by direct cooling to -4 ℃. Sterilized artificial tap water in vermiculite was conducive to anhydrobiosis of HBN. The ability of HBN infecting Galleria mellonella was enhanced in the short term freezing storage in seven days. |
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