| 沈一凡, 沈慧敏* , 岳秀利, 郭金梅, 宋丽雯.二斑叶螨抗阿维菌素品系选育及其解毒酶系活力变化[J].植物保护,2014,40(5):44-48. |
| 二斑叶螨抗阿维菌素品系选育及其解毒酶系活力变化 |
| Resistance selection of Tetranychus urticae to abamectin strain and changes in the activity of detoxification enzymes |
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| DOI： |
| 中文关键词: 二斑叶螨 阿维菌素 抗性选育 解毒酶 抗性机理 |
| 英文关键词:Tetranychus urticae abamectin resistance selection detoxification enzyme biochemical mechanism of resistance |
| 基金项目:公益性行业(农业)科研专项(201103020); 国家自然科学基金(31260442) |
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| 中文摘要: |
| 采用室内生物测定和生化分析方法, 以采自甘肃兰州国家级森林公园兴隆山的二斑叶螨为敏感品系(SS), 研究二斑叶螨对阿维菌素的抗药性及抗性生化机理。结果表明：在室内用阿维菌素强化筛选24代, 获得了二斑叶螨抗阿维菌素品系(Ab-R24), 抗性指数(resistance index, RI)为321.5。对SS和Ab-R24解毒酶活性的分析表明, Ab-R24品系体内羧酸酯酶(CarE)、乙酰胆碱酯酶(AchE)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(ALP)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)活性分别是SS品系的1.43、1.18、1.56、1.48、1.55倍和4.02倍, 差异达到显著水平(P < 0.05), 其中MFO的活性上升最为显著。对SS和Ab-R-24解毒酶动力学常数的分析表明, Ab-R-24品系体内AchE、GSTs和MFO的 Km分别是SS品系的1.14、2.31倍和2.58倍; Vmax分别是SS品系的1.19、2.34倍和1.76倍, 差异均达到显著水平(P <0.05)。说明二斑叶螨对阿维菌素抗性增高与MFO活性快速升高有关, AchE和GSTs也参与阿维菌素抗性的形成。 |
| 英文摘要: |
| In order to study the insecticide resistance and resistance biochemistry mechanism of Tetranychus urticae to abamectin, we used bioassay and biochemical analysis methods. In addition, T. urticae from Xinglong Mountain in Gansu Lanzhou National Forest Park was used as a susceptible strain (SS). The results indicated that T. urticae abamectin-resistance 24 (Ab-R-24) was obtained after indoor screening for 24 generations with abamectin, whose resistance index (RI) was 321.5. The analysis of SS and Ab-R24 detoxification enzyme activity showed that the activities of carboxylesterases (CarE), acetylcholinesterase (AchE), acid phosphatase (ACP), alkaline phosphatase (ALP), glutathione S-transferase (GSTs) and mixed function oxidase (MFO) of Ab-R24 strain were in vivo 1.43, 1.18, 1.56, 1.48, 1.55 and 4.02 times of the corresponding activity of SS, respectively, which reached the significance level ( P <0.05). The analysis of SS and Ab-R24 detoxifying enzyme kinetic constants showed that the Km values of AchE/GSTs and MFO of Ab-R-24 strain were in vivo 1.14, 2.31 and 2.58 times of the corresponding value of SS, respectively; at the same time, the Vmax values were 1.19, 2.34 and 1.76 times of the corresponding value of SS, respectively, which reached the significance level (P <0.05). In conclusion, the activity of MFO rose most obviously, indicating that the rise of T. urticae’s abamectin resistance was closely related with the rapid rise of MFO’s activity. There is no doubt that AchE and GSTs are both involved in the formation of abamectin resistance. |
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