| 陈泰祥, 陈秀蓉*, 杨成德, 朱海波, 王涵琦, 卞静.甘肃省马铃薯束梗褐腐病病原鉴定及其生物学特性研究[J].植物保护,2014,40(5):27-33. |
| 甘肃省马铃薯束梗褐腐病病原鉴定及其生物学特性研究 |
| Identification and biological characteristics of Solanum tuberosum brown rot caused by Doratomyces stemonitis |
| |
| DOI： |
| 中文关键词: 马铃薯 细基束梗霉 病原分离鉴定 生物学特性 |
| 英文关键词:Solanum tuberosum Doratomyces stemonitis pathogen identification biological characteristics |
| 基金项目:甘肃省农牧厅项目 |
|
| 摘要点击次数: 1423 |
| 全文下载次数: 1554 |
| 中文摘要: |
| 对甘肃省马铃薯(Solanum tuberosum L.)束梗褐腐病病原进行了分离鉴定和生物学特性研究。经形态特征观察与rDNA-ITS序列分析, 将病原菌鉴定为细基束梗霉［Doratomyces stemonitis (Pers. ex Fr.) F.J. Morton & G. Smith］。生物学特性研究结果表明, 病菌菌丝生长最适温度为25～30 ℃, 最适pH为6, 病菌能利用多种碳源, 但以蔗糖最好, 氮源以甘氨酸最适, 光照对菌丝生长没有影响。分生孢子在5～40 ℃范围内均能萌发, 最适25 ℃, 最适pH为7, 分生孢子萌发需液态水, 湿度低于99%几乎不萌发, 马铃薯汁液和葡萄糖液对孢子萌发有较好的促进作用。本研究为马铃薯束梗褐腐病的防治提供了理论依据。 |
| 英文摘要: |
| The identification and biological characteristics of the pathogen isolated from brown rot of Solanum tuberosum were studied. The pathogen was identified as Doratomyces stemonitis (Pers. ex Fr.) F.J. Morton & G. Smith based on morphological characteristics as well as rDNA-ITS sequences. The optimum temperature for mycelial growth was 25-30 ℃ at pH 6. The pathogen could use monosaccharide, disaccharide and polysaccharide; the best carbon source was sucrose, and the best nitrogen source was glycine. Illumination had no obvious promotion effect on mycelial growth. The optimum temperature for sporangium sprout was 25 ℃ at pH 7. Spores could germinate only in water drop. The filtrate of potato and glucose solution had obvious promotion effect on spore germination. Soil solution had no effects on spore germination. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
